Anti-Hu BCL2 FITC

Anti-Hu BCL2 FITC
Regulatory status
RUO
Antigen
BCL2
Clone
Bcl-2/100
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-668-T100
In stock

25 tests
1F-668-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Negative species
Mouse
Immunogen
Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
Other names
Bcl-2, PPP1R50
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1F-668_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1F-668_FC_Histogram
Separation of human BCL2 positive lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Joubert A, Marais S, Maritz C: Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels. Biocell. 2009 Apr;33(1):67-70.
PubMed
Laforge M, Petit F, Estaquier J, Senik A: Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef. J Virol. 2007 Oct;81(20):11426-40.
PubMed
Laflamme C, Israël-Assayag E, Cormier Y: Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis. Eur Respir J. 2003 Feb;21(2):225-31.
PubMed
Soilu-Hänninen M, Ekert P, Bucci T, Syroid D, Bartlett PF, Kilpatrick TJ: Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway. J Neurosci. 1999 Jun 15;19(12):4828-38.
PubMed
Gugasyan R, Christou A, O'Reilly LA, Strasser A, Gerondakis S: Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA. Cell Death Differ. 2006 Jul;13(7):1235-7.
PubMed
Variant
100 tests
1F-668-T100
In stock

25 tests
1F-668-T025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock