Exbio — Research products — Antibodies — Other intracellular antigens — Anti-H-RAS Purified

Anti-H-RAS Purified

Regulatory status

RUO

Antigen

H-RAS

Clone

H-RAS-03

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-527-C100

In stock

150.00 USD

0.025 mg

11-527-C025

Delivery 1 week

75.00 USD

Variant

0.1 mg

11-527-C100

In stock

150.00 USD

0.025 mg

11-527-C025

Delivery 1 week

75.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.

Application

Application details

Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Reactivity

Human

Immunogen

Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ras-H, HRAS, p21Ras

Antigen description

H-Ras is one of three ubiquitously isoforms of Ras GTPase that operate at the intracellular leaflet of the plasma membrane to regulate multiple signal transduction pathways, such as mitogen-activated protein kinase (MAPK) cascade. H-Ras is anchored to the plasma membrane by farnesyl and two palmityl residues. GTP loading decreases H-Ras affinity for lipid rafts and allows the protein to target to nonraft microdomains, the primary sites of H-Ras signaling. Sos protein and other guanine nucleotide-exchange factors catalyze dissociation of GDP from Ras. Besides its roles in the plasma membrane, active H-Ras also diffuses through the cytoplasm on nanoparticles termed rasosomes, which is dependent on Ras palmitoylation.

Entrez Gene ID 3265

UniProt ID P01112

Western blotting analysis of human H-Ras using mouse monoclonal antibody H-Ras-03 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-H-Ras monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for H-Ras at approximately 19 kDa.

General references:

Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y: Ras and its signals diffuse through the cell on randomly moving nanoparticles. Cancer Res. 2006 Feb 15;66(4):1974-81.
PubMed

Roy S, Plowman S, Rotblat B, Prior IA, Muncke C, Grainger S, Parton RG, Henis YI, Kloog Y, Hancock JF: Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling. Mol Cell Biol. 2005 Aug;25(15):6722-33.
PubMed

Zheng Y, Quilliam LA: Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO Rep. 2003 May;4(5):463-8.
PubMed

Prior IA, Harding A, Yan J, Sluimer J, Parton RG, Hancock JF: GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001 Apr;3(4):368-75.
PubMed

Rotblat B, Prior IA, Muncke C, Parton RG, Kloog Y, Henis YI, Hancock JF: Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol Cell Biol. 2004 Aug;24(15):6799-810.
PubMed