Anti-H-RAS Purified

Anti-H-RAS Purified
Regulatory status
RUO
Antigen
H-RAS
Clone
H-RAS-03
Format
Purified
Reactivity
Human
Application
WB
Variant
0.1 mg
11-527-C100
In stock

0.025 mg
11-527-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody H-RAS-03 reacts with human H-Ras, an ubiquitously expressed 21 kDa intracellular protein. Although reactivity with other species has not been determined, it is probable as the epitope is highly conserved among animals.
Application
WB
Reactivity
Human
Immunogen
Peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras protein
Other names
RAS-H, HRAS, p21Ras
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-527 WB
Western blotting analysis (reducing conditions) of H-Ras in whole cell lysate using anti-H-Ras (H-RAS-03). Lane 1: HeLa human cervix carcinoma cell line Lane 2: K567 human leukemia cell line Lane 3: RAJI human Burkitt lymphoma cell line

General references:

Rotblat B, Yizhar O, Haklai R, Ashery U, Kloog Y: Ras and its signals diffuse through the cell on randomly moving nanoparticles. Cancer Res. 2006 Feb 15;66(4):1974-81.
PubMed
Roy S, Plowman S, Rotblat B, Prior IA, Muncke C, Grainger S, Parton RG, Henis YI, Kloog Y, Hancock JF: Individual palmitoyl residues serve distinct roles in H-ras trafficking, microlocalization, and signaling. Mol Cell Biol. 2005 Aug;25(15):6722-33.
PubMed
Zheng Y, Quilliam LA: Activation of the Ras superfamily of small GTPases. Workshop on exchange factors. EMBO Rep. 2003 May;4(5):463-8.
PubMed
Prior IA, Harding A, Yan J, Sluimer J, Parton RG, Hancock JF: GTP-dependent segregation of H-ras from lipid rafts is required for biological activity. Nat Cell Biol. 2001 Apr;3(4):368-75.
PubMed
Rotblat B, Prior IA, Muncke C, Parton RG, Kloog Y, Henis YI, Hancock JF: Three separable domains regulate GTP-dependent association of H-ras with the plasma membrane. Mol Cell Biol. 2004 Aug;24(15):6799-810.
PubMed
Variant
0.1 mg
11-527-C100
In stock

0.025 mg
11-527-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock