Exbio — Research products — Antibodies — Other intracellular antigens — Anti-GRB2 Purified

Anti-GRB2 Purified

Regulatory status

RUO

Antigen

GRB2

Clone

PAb (554)

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-554-C100

In stock

198.00 USD

0.025 mg

11-554-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-554-C100

In stock

198.00 USD

0.025 mg

11-554-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rabbit polyclonal

Specificity

The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa intracellular adaptor protein. The immunogen has 100% identity between man, cow, rat and mouse.

Application

Application details

Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Reactivity

Human

Immunogen

Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ASH, NCKAP2

Antigen description

Grb2 (growth factor receptor-bound protein 2) is a highly conserved adaptor protein composed of central SH2 domain flanked by two terminal SH3 domains. Grb2 mediates coupling of receptor protein tyrosine kinases to a guanine nucleotide exchange factor Sos, thereby enabling the tyrosine kinases to stimulate activity of Ras and hence of MAP kinase pathway. Grb2 also can bind to receptor protein tyrosine phosphatases and affect their signaling.

Entrez Gene ID 2885

UniProt ID P62993

Western bloting analysis of human GRB2 using rabbit polyclonal antibody PAb (554) on lysates of MOLT-4 and RAMOS cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-GRB2 antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for GRB2 at approximately 24 kDa.

General references:

Sieh M, Batzer A, Schlessinger J, Weiss A: GRB2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation. Mol Cell Biol. 1994 Jul;14(7):4435-42.
PubMed

Reif K, Buday L, Downward J, Cantrell DA: SH3 domains of the adapter molecule Grb2 complex with two proteins in T cells: the guanine nucleotide exchange protein Sos and a 75-kDa protein that is a substrate for T cell antigen receptor-activated tyrosine kinases. J Biol Chem. 1994 May 13;269(19):14081-7.
PubMed

den Hertog J, Hunter T: Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 1996 Jun 17;15(12):3016-27.
PubMed