Anti-GRB2 Purified

Anti-GRB2 Purified
Regulatory status
RUO
Antigen
GRB2
Clone
PAb (554)
Format
Purified
Reactivity
Human
Application
WB
Variant
0.1 mg
11-554-C100
In stock

0.025 mg
11-554-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa adaptor protein. The immunogen has 100% identity between man, cow, rat and mouse.
Application
WB
Reactivity
Human
Immunogen
Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).
Other names
ASH, NCKAP2
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-554 WB
Western blotting analysis (reducing conditions) of Ramos cell lysate using two batches of rabbit anti-Grb2 polyclonal (A, B).

General references:

Sieh M, Batzer A, Schlessinger J, Weiss A: GRB2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation. Mol Cell Biol. 1994 Jul;14(7):4435-42.
PubMed
Reif K, Buday L, Downward J, Cantrell DA: SH3 domains of the adapter molecule Grb2 complex with two proteins in T cells: the guanine nucleotide exchange protein Sos and a 75-kDa protein that is a substrate for T cell antigen receptor-activated tyrosine kinases. J Biol Chem. 1994 May 13;269(19):14081-7.
PubMed
den Hertog J, Hunter T: Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 1996 Jun 17;15(12):3016-27.
PubMed
Variant
0.1 mg
11-554-C100
In stock

0.025 mg
11-554-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock