Exbio — Research products — Antibodies — Other intracellular antigens — Anti-GRB2 Purified

Anti-GRB2 Purified

Anti-GRB2 Purified
Regulatory status
RUO
Antigen
GRB2
Clone
PAb (554)
Format
Purified
Reactivity
Human
Application
WB
Variant
0.1 mg
11-554-C100
In stock
180.00 USD
0.025 mg
11-554-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-554-C100
In stock
180.00 USD
0.025 mg
11-554-C025
Delivery 1 week
90.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kDa intracellular adaptor protein. The immunogen has 100% identity between man, cow, rat and mouse.
Application
WB
Application details
Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.
Reactivity
Human
Immunogen
Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).
Concentration
1 mg/ml
Preparation
Purified by ligand affinity chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ASH, NCKAP2
Antigen description
Grb2 (growth factor receptor-bound protein 2) is a highly conserved adaptor protein composed of central SH2 domain flanked by two terminal SH3 domains. Grb2 mediates coupling of receptor protein tyrosine kinases to a guanine nucleotide exchange factor Sos, thereby enabling the tyrosine kinases to stimulate activity of Ras and hence of MAP kinase pathway. Grb2 also can bind to receptor protein tyrosine phosphatases and affect their signaling.
Entrez Gene ID 2885
UniProt ID P62993
11-554_WB
Western bloting analysis of human GRB2 using rabbit polyclonal antibody PAb (554) on lysates of MOLT-4 and RAMOS cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-GRB2 antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for GRB2 at approximately 24 kDa.

General references:

Sieh M, Batzer A, Schlessinger J, Weiss A: GRB2 and phospholipase C-gamma 1 associate with a 36- to 38-kilodalton phosphotyrosine protein after T-cell receptor stimulation. Mol Cell Biol. 1994 Jul;14(7):4435-42.
PubMed
Reif K, Buday L, Downward J, Cantrell DA: SH3 domains of the adapter molecule Grb2 complex with two proteins in T cells: the guanine nucleotide exchange protein Sos and a 75-kDa protein that is a substrate for T cell antigen receptor-activated tyrosine kinases. J Biol Chem. 1994 May 13;269(19):14081-7.
PubMed
den Hertog J, Hunter T: Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 1996 Jun 17;15(12):3016-27.
PubMed
Variant
0.1 mg
11-554-C100
In stock
180.00 USD
0.025 mg
11-554-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-554-C100
In stock
180.00 USD
0.025 mg
11-554-C025
Delivery 1 week
90.00 USD
Contact distributor
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