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Exbio
—
Research products
—
Antibodies
—
Other intracellular antigens
—
Anti-GRAP2 APC
Anti-GRAP2 APC
Regulatory status
RUO
Antigen
GRAP2
Clone
UW40
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
0.1 mg
1A-639-C100
In stock
242.00 USD
Variant
0.1 mg
1A-639-C100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
GST-fusion human GRAP2/GADS protein
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GADS, GRID, GRAP-2, GrbX
Antigen description
GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.
Entrez Gene ID
9402
UniProt ID
O75791
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human GRAP2(UW40) APC antibody (concentration in sample 1,7 μg/ml).
Separation of lymphocytes stained using anti-human GRAP2 (UW40) APC antibody (concentration in sample 1,7 μg/ml, red-filled) from lymphocytes stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 1,7 μg/ml, same as GRAP2 APC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of peripheral blood.
General references:
Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in CD28-mediated costimulation. J Immunol. 2006 Jul 15;177(2):1085-91.
PubMed
Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full-length adaptor protein GADS in solution determined using small-angle X-ray scattering. Biophys J. 2008 Mar 1;94(5):1766-72.
PubMed
Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage. Eur J Immunol. 2009 May;39(5):1395-404.
PubMed
Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads. EMBO J. 2004 Apr 7;23(7):1441-51
PubMed
Product specific references:
Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J Immunol. 2004 Aug 1;173(3):1711-20.
PubMed
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6789-93.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1A-639-C100
In stock
242.00 USD
Variant
0.1 mg
1A-639-C100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
1A-724-C100
Mouse IgG2a Isotype Control APC
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Clone
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Reg. status
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Application
FC, FC-IC
0.1 mg
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