Exbio — Research products — Antibodies — Other intracellular antigens — Anti-GRAP2 APC

Anti-GRAP2 APC

Regulatory status

RUO

Antigen

GRAP2

Clone

UW40

Format

APC

Reactivity

Human

Application

FC-IC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-639-C100

In stock

248.60 USD

Variant

0.1 mg

1A-639-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

Application

FC-IC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

GST-fusion human GRAP2/GADS protein

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GADS, GRID, GRAP-2, GrbX

Antigen description

GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.

Entrez Gene ID 9402

UniProt ID O75791

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human GRAP2(UW40) APC antibody (concentration in sample 1,7 μg/ml).

Separation of lymphocytes stained using anti-human GRAP2 (UW40) APC antibody (concentration in sample 1,7 μg/ml, red-filled) from lymphocytes stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 1,7 μg/ml, same as GRAP2 APC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of peripheral blood.

General references:

Watanabe R, Harada Y, Takeda K, Takahashi J, Ohnuki K, Ogawa S, Ohgai D, Kaibara N, Koiwai O, Tanabe K, Toma H, Sugamura K, Abe R: Grb2 and Gads exhibit different interactions with CD28 and play distinct roles in CD28-mediated costimulation. J Immunol. 2006 Jul 15;177(2):1085-91.
PubMed

Moran O, Roessle MW, Mariuzza RA, Dimasi N: Structural features of the full-length adaptor protein GADS in solution determined using small-angle X-ray scattering. Biophys J. 2008 Mar 1;94(5):1766-72.
PubMed

Dalheimer SL, Zeng L, Draves KE, Hassaballa A, Jiwa NN, Parrish TD, Clark EA, Yankee TM: Gads-deficient thymocytes are blocked at the transitional single positive CD4+ stage. Eur J Immunol. 2009 May;39(5):1395-404.
PubMed

Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA: Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads. EMBO J. 2004 Apr 7;23(7):1441-51
PubMed

Product specific references:

Yankee TM, Yun TJ, Draves KE, Ganesh K, Bevan MJ, Murali-Krishna K, Clark EA: The Gads (GrpL) adaptor protein regulates T cell homeostasis. J Immunol. 2004 Aug 1;173(3):1711-20.
PubMed

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed

Yankee TM, Draves KE, Ewings MK, Clark EA, Graves JD: CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling. Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6789-93.
PubMed