Exbio — Research products — Antibodies — Other intracellular antigens — Anti-GAPDHS Purified

Anti-GAPDHS Purified

Regulatory status

RUO

Antigen

GAPDHS

Clone

Hs-8

Format

Purified

Reactivity

Pig, Human

Application

WB, FC (QC tested), ICC

Variant

0.1 mg

11-301-C100

In stock

176.00 USD

0.025 mg

11-301-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-301-C100

In stock

176.00 USD

0.025 mg

11-301-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Application

WB, FC (QC tested), ICC

Application details

Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining.

Reactivity

Pig, Human

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

SGAPDH, HSD-35

Antigen description

GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Entrez Gene ID 26330

UniProt ID O14556

Flow cytometry intracellular staining pattern of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.

Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.

General references:

Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
PubMed

Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32.
PubMed

Teplá O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).

Product specific references:

Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J: Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm. Reprod Biol Endocrinol. 2015 Mar 8;13:15. doi: 10.1186/s12958-015-0008-1.
PubMed