Exbio — Research products — Antibodies — Other intracellular antigens — Anti-GAPDHS Purified

Anti-GAPDHS Purified

Anti-GAPDHS Purified
Regulatory status
RUO
Antigen
GAPDHS
Clone
Hs-8
Format
Purified
Reactivity
Pig, Human
Application
WB, FC (QC tested), ICC
Variant
0.1 mg
11-301-C100
In stock
160.00 USD
0.025 mg
11-301-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-301-C100
In stock
160.00 USD
0.025 mg
11-301-C025
Delivery 1 week
80.00 USD
Contact distributor
Product details
Description
References
Isotype
Mouse IgM
Specificity
The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
Application
WB, FC (QC tested), ICC
Application details
Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining.
Reactivity
Pig, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
SGAPDH, HSD-35
Antigen description
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Entrez Gene ID 26330
UniProt ID O14556

General references:

Peknicova J, Kyselova V, Buckiova D, Boubelik M: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.
PubMed
Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32.
PubMed
Teplá O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).

Product specific references:

Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J: Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm. Reprod Biol Endocrinol. 2015 Mar 8;13:15. doi: 10.1186/s12958-015-0008-1.
PubMed
Variant
0.1 mg
11-301-C100
In stock
160.00 USD
0.025 mg
11-301-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-301-C100
In stock
160.00 USD
0.025 mg
11-301-C025
Delivery 1 week
80.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product