Anti-FYN Purified

Anti-FYN Purified
Regulatory status
RUO
Antigen
FYN
Clone
FYN-01
Format
Purified
Reactivity
Human, Mouse
Variant
0.1 mg
11-375-C100
In stock
180.00 USD

0.025 mg
11-375-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: brain.
Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line.
Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
Reactivity
Human, Mouse
Immunogen
Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
p59Fyn, SLK, SYN
Antigen description
Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g. in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptor functions, thus affecting various brain functions, and even many of its other substrates are important for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axon growth and guidance. In immune system Fyn namely regulates the commitment of T cells to activation, is important in T cell anergy induction, promotes mast cell chemotaxis and reorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved in embryonic stem cell growth and differentiation, associates with tubulin and may play roles in mitotic spindle formation.
Entrez Gene ID 2534
UniProt ID P06241
11-375 IP
Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.
11-375_ICC
Immunocytochemistry analysis of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Primary antibody: 5 ug/ml
11-375_ICC_2
Immunocytochemistry analysis of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
11-375_ICC_3
Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).

General references:

Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20.
PubMed
Makinodan E, Manabe T, Makinodan M, Yamauchi T, Matsuyoshi H, Sakumura R, Tatsumi K, Wanaka A. A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells. Neuroscience. 2007 Jun 15;147(1):1-4.
PubMed
Samayawardhena LA, Kapur R, Craig AW: Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells. Blood. 2007 May 1;109(9):3679-86.
PubMed
Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. Fyn regulates the duration of TCR engagement needed for commitment to effector function. J Immunol. 2007 Oct 1;179(7):4635-44.
PubMed
Wu HY, Hsu FC, Gleichman AJ, Baconguis I, Coulter DA, Lynch DR: Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems. J Biol Chem. 2007 Jul 13;282(28):20075-87.
PubMed
Hattori K, Uchino S, Isosaka T, Maekawa M, Iyo M, Sato T, Kohsaka S, Yagi T, Yuasa S: Fyn is required for haloperidol-induced catalepsy in mice. J Biol Chem. 2006 Mar 17;281(11):7129-35.
PubMed
Talmor-Cohen A, Tomashov-Matar R, Tsai WB, Kinsey WH, Shalgi R: Fyn kinase-tubulin interaction during meiosis of rat eggs. Reproduction. 2004 Oct;128(4):387-93.
PubMed
Utting O, Priatel JJ, Teh SJ, Teh HS: p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation. J Immunol. 2001 Feb 1;166(3):1540-6.
PubMed

Product specific references:

Meraner P, Horejsí V, Wolpl A, Fischer GF, Stingl G, Maurer D: Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation. J Immunol. 2007 Feb 15;178(4):2262-71.
PubMed
Variant
0.1 mg
11-375-C100
In stock
180.00 USD

0.025 mg
11-375-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD