Exbio — Research products — Antibodies — Other intracellular antigens — Anti-EGFR PE-Cy™7

Anti-EGFR PE-Cy™7

Regulatory status

RUO

Antigen

EGFR

Clone

EGFR1

Format

PE-Cy™7

Reactivity

Horse, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-680-T100

In stock

275.00 USD

25 tests

T7-680-T025

In stock

137.50 USD

Variant

100 tests

T7-680-T100

In stock

275.00 USD

25 tests

T7-680-T025

In stock

137.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody EGFR1 reacts with extracellular domain of human protein kinase EGFR (ErbB1 / HER1); epitope within amino acids 6-273.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Horse, Human

Negative species

Mouse, Rat

Immunogen

Human epidermoid carcinoma line A431

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

EGF-R, Her1, ERBB1, PIG61, NISBD2

Antigen description

The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.

Entrez Gene ID 1956

UniProt ID P00533

Separation of A431 cells stained using anti-EGFR (EGFR1) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml, red-filled) from A431 cells stained using mouse IgG1 isotype control (MOPC-21) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml, same as EGFR PE-Cy™7 concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Fortunel NO, Hatzfeld JA, Rosemary PA, Ferraris C, Monier MN, Haydont V, Longuet J, Brethon B, Lim B, Castiel I, Schmidt R, Hatzfeld A: Long-term expansion of human functional epidermal precursor cells: promotion of extensive amplification by low TGF-beta1 concentrations. J Cell Sci. 2003 Oct 1;116(Pt 19):4043-52.
PubMed

Aigner A, Juhl H, Malerczyk C, Tkybusch A, Benz CC, Czubayko F: Expression of a truncated 100 kDa HER2 splice variant acts as an endogenous inhibitor of tumour cell proliferation. Oncogene. 2001 Apr 19;20(17):2101-11.
PubMed

Akiyama M, Smith LT, Shimizu H: Changing patterns of localization of putative stem cells in developing human hair follicles. J Invest Dermatol. 2000 Feb;114(2):321-7.
PubMed

Rebetz J, Tian D, Persson A, Widegren B, Salford LG, Englund E, Gisselsson D, Fan X: Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma. PLoS One. 2008 Apr 9;3(4):e1936.
PubMed