Exbio — Research products — Antibodies — Other intracellular antigens — Anti-CSK Purified

Anti-CSK Purified

Regulatory status

RUO

Antigen

CSK

Clone

CSK-04

Format

Purified

Reactivity

Human, Mouse

Application

IP, WB, ICC

Variant

0.1 ml

11-378-R100

In stock

198.00 USD

0.025 ml

11-378-R025

Delivery 1 week

99.00 USD

Variant

0.1 ml

11-378-R100

In stock

198.00 USD

0.025 ml

11-378-R025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa intracellular non-receptor protein tyrosine kinase.

Application

IP, WB, ICC

Application details

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line.
Western blotting: The recommended dilution of the antibody CSK-04 is 1:250-1:300; weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions.
Immunoprecipitation: The immunosorbent (CSK-04 antibody covalently bound to beads) immunoprecipitates Csk from cell lysates.

Reactivity

Human, Mouse

Immunogen

Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them it lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine. Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of those Src-family kinases, that are localized in lipid rafts, is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.

Entrez Gene ID 1445

UniProt ID P41240

Immunocytochemistry staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

General references:

Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3 Phosphatase. J Biol Chem. 2008 Apr 18;283(16):10339-46.
PubMed

Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
PubMed

Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
PubMed

Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
PubMed

Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
PubMed

Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.
PubMed

Product specific references:

Palomero T, Couronné L, Khiabanian H, Kim MY, Ambesi-Impiombato A, Perez-Garcia A, Carpenter Z, Abate F, Allegretta M, Haydu JE, Jiang X, Lossos IS, Nicolas C, Balbin M, Bastard C, Bhagat G, Piris MA, Campo E, Bernard OA, Rabadan R, Ferrando AA: Recurrent mutations in epigenetic regulators, RHOA and FYN kinase in peripheral T cell lymphomas. Nat Genet. 2014 Feb;46(2):166-70.
PubMed