Exbio — Research products — Antibodies — Other intracellular antigens — Anti-Can Intra-Acrosomal Protein Purified

Anti-Can Intra-Acrosomal Protein Purified

Regulatory status

RUO

Antigen

Intra-Acrosomal Protein

Clone

Ds-2

Format

Purified

Reactivity

Dog

Application

WB, ICC

Variant

0.1 mg

11-281-C100

Ask for delivery term HERE

176.00 USD

0.025 mg

11-281-C025

Delivery 1 week

88.00 USD

Variant

0.1 mg

11-281-C100

Ask for delivery term HERE

176.00 USD

0.025 mg

11-281-C025

Delivery 1 week

88.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Mouse IgG

Specificity

The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.

Application

WB, ICC

Application details

Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Reactivity

Dog

Immunogen

Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IAP Can

Antigen description

One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Product specific references:

Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia. 1997 Sep-Oct;29(5):261-8.
PubMed