Anti-Can Intra-Acrosomal Protein Purified

Anti-Can Intra-Acrosomal Protein Purified
Regulatory status
RUO
Antigen
Intra-Acrosomal Protein
Clone
Ds-2
Format
Purified
Reactivity
Dog
Application
Variant
0.1 mg
11-281-C100
In stock
160.00 USD

0.025 mg
11-281-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Description
References
Isotype
Mouse IgG
Specificity
The mouse monoclonal antibody Ds-2 reacts with canine intra-acrosomal sperm protein (92 and 42 kDa), a testis-specific acrosomal vesicle protein 1 associated with the acrosomal membranes and matrix of the mature sperm.
Application
Application details
Immunocytochemistry: Membrane permeabilization (acetone) is essential.
Reactivity
Dog
Immunogen
Freshly ejaculated dog sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid, lyophylized and subsequently used for immunization.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
IAP Can
Antigen description
One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Product specific references:

Geussova G, Peknicova J, Capkova J, Kalab P, Moos J, Philimonenko VV, Hozak P: Monoclonal antibodies to canine intra-acrosomal sperm proteins recognizing acrosomal status during capacitation and acrosome reaction. Andrologia. 1997 Sep-Oct;29(5):261-8.
PubMed
Variant
0.1 mg
11-281-C100
In stock
160.00 USD

0.025 mg
11-281-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD