Exbio — Research products — Antibodies — Other intracellular antigens — Anti-ARHGEF4 Purified

Anti-ARHGEF4 Purified

Regulatory status

RUO

Antigen

ARHGEF4

Clone

ARHGEF-08

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-723-C100

Ask for delivery term HERE

165.00 USD

0.025 mg

11-723-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-723-C100

Ask for delivery term HERE

165.00 USD

0.025 mg

11-723-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ASEF1, GEF4, STM6

Antigen description

ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli – stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.

Entrez Gene ID 50649

UniProt ID Q9NR80

Western blotting analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).

Separation of cells stained using anti-ARHGEF4 (ARHGEF-08) purified antibody (concentration in sample 4 μg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining) of ARHGEF4 transfected HEK-293 cell suspension.

General references:

Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb;22(2):372-86.
PubMed

Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan;34(1):109-15.
PubMed

Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. J Biol Chem. 2009 Aug 14;284(33):22436-43.
PubMed