Anti-ARHGEF4 PE

Anti-ARHGEF4 PE
Regulatory status
RUO
Antigen
ARHGEF4
Clone
ARHGEF-08
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-723-C100
Out of stock
220.00 USD

0.025 mg
1P-723-C025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.
Application
FC (QC tested)
Reactivity
Human
Immunogen
Recombinant fragment of human ARHGEF4 (amino acids 143-271)
Other names
ASEF1, GEF4, STM6
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-723 FC
Flow cytometry intracellular staining pattern of of ARHGEF4 in transfected HEK-293 cells stained using anti-ARHGEF4 (ARHGEF-08) PE antibody (concentration in sample 1 μg/ml, blue) and Mouse IgG1 Isotype Control (MOPC-21) PE antibody (concentration in sample 1 μg/ml, orange).

General references:

Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G: Structural basis for the recognition of Asef by adenomatous polyposis coli. Cell Res. 2012 Feb;22(2):372-86.
PubMed
Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J: The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation. Leuk Res. 2010 Jan;34(1):109-15.
PubMed
Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T: Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase. J Biol Chem. 2009 Aug 14;284(33):22436-43.
PubMed
Variant
0.1 mg
1P-723-C100
Out of stock
220.00 USD

0.025 mg
1P-723-C025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD