Anti-AKT1 Purified

Anti-AKT1 Purified
Regulatory status
RUO
Antigen
AKT1
Clone
PAb (509)
Format
Purified
Reactivity
Human
Application
Variant
0.05 mg
11-509-C050
In stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD
Product details
Description
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody reacts with Akt1 (intracellular antigen; recognized epitope between aa 85-100). Akt/PKB protein kinases family has a three mammalian members identified to date (Akt1, Akt2, Akt3). Human Akt1 has a predicted molecular weight of 55.7 kDa.
Application
Application details
Western blotting: Recommended dilution: 0.5-1 μg/ml; positive material: RAJI human Burkitt lymphoma cell line; both reducing and non-reducing conditions, reducing conditions are recommended.
Reactivity
Human
Immunogen
Synthetic peptide (coupled with KLH) derived from aminoacid sequence 85-100 of human Akt1. This immunogen sequence has 100% homology with mouse and rat Akt1/PKB.
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by affinity chromatography
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
RAC-PK-alpha, Protein kinase B, PKB
Antigen description
Serine/threonine protein kinase Akt/PKB (protein kinase B) plays important roles in cell growth, metabolism, proliferation and survival. Upon extracellular stimulus that leads to activation of phosphatidylinositol 3-kinase (PI3K), Akt is recruited from the cytosol to the plasma membrane through binding 3-phosphoinositides and is phosphorylated. Active Akt dissociates from the plasma membrane to gain access to various substrates in different subcellular locations by translocating to cytosol and nucleus.
Entrez Gene ID 207
UniProt ID P31749

General references:

Ananthanarayanan B, Fosbrink M, Rahdar M, Zhang J: Live-cell molecular analysis of AKT activation reveals roles for activation loop phosphorylation. J Biol Chem. 2007 Oct 10.
PubMed
Variant
0.05 mg
11-509-C050
In stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD