Exbio — Research products — Antibodies — Other intracellular antigens — Anti-AGPS Purified

Anti-AGPS Purified

Regulatory status

RUO

Antigen

AGPS

Clone

AGPS-03

Format

Purified

Reactivity

Human

Application

FC(IC), WB

Variant

0.1 mg

11-739-C100

Ask for delivery term HERE

180.00 USD

0.025 mg

11-739-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

11-739-C100

Ask for delivery term HERE

180.00 USD

0.025 mg

11-739-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody AGPS-03 recognizes AGPS (alkykglycerone phosphate synthase), an intracellular peroxisomal enzyme important for lipid biosynthesis.

Application

FC(IC), WB

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Human

Immunogen

recombinant human AGPS (amino acids 158-384)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ADAS, ADPS, RCDP3, ADAP-S, ADHAPS, ALDHPSY

Antigen description

AGPS (alkylglycerone phosphate synthase), is an enzyme that catalyzes the second step of ether lipid biosynthesis in which acyl-dihydroxyacetone phosphate (acyl-DHAP) is converted to alkyl-DHAP by addition of a long chain alcohol and removal of a long-chain acid anion. The protein is localized to the inner side of the peroxisomal membrane and requires FAD as a cofactor. Mutations in AGPS gene have been associated with type 3 of rhizomelic chondrodysplasia punctata (RCDP3), and Zellweger syndrome. Higher expression of AGPS was observed in BCR/ABL positive leukemias and it was also described to be associated with higher risk of relapse.

Entrez Gene ID 8540

UniProt ID O00116

Western blotting analysis of human AGPS using mouse monoclonal antibody AGPS-03 on lysates of HEP-G2 and Molt-4 cells, and MCF-7 cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-AGPS monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. AGPS was detected at approximately 70 kDa.

Separation of K562 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with K562 cells stained using anti-AGPS (AGPS-03) purified antibody (concentration in sample 5 μg/ml, GAM APC).

General references:

Itzkovitz B, Jiralerspong S, Nimmo G, Loscalzo M, Horovitz DD, Snowden A, Moser A, Steinberg S, Braverman N: Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. Hum Mutat. 2012 Jan;33(1):189-97
PubMed

Grimm MO, Kuchenbecker J, Rothhaar TL, Grösgen S, Hundsdörfer B, Burg VK, Friess P, Müller U, Grimm HS, Riemenschneider M, Hartmann T: Plasmalogen synthesis is regulated via alkyl-dihydroxyacetonephosphate-synthase by amyloid precursor protein processing and is affected in Alzheimer's disease. J Neurochem. 2011 Mar;116(5):916-25.
PubMed

Bhojwani D, Kang H, Menezes RX, Yang W, Sather H, Moskowitz NP, Min DJ, Potter JW, Harvey R, Hunger SP, Seibel N, Raetz EA, Pieters R, Horstmann MA, Relling MV, den Boer ML, Willman CL, Carroll WL: Gene expression signatures predictive of early response and outcome in high-risk childhood acute lymphoblastic leukemia: A children's oncology group study. J Clin Oncol. 2008 Sep 20;26(27):4376-84.
PubMed

Yeoh EJ, Ross ME, Shurtleff SA, Williams WK, Patel D, Mahfouz R, Behm FG, Raimondi SC, Relling MV, Patel A, Cheng C, Campana D, Wilkins D, Zhou X, Li J, Liu H, Pui CH, Evans WE, Naeve C, Wong L, Downing JR: Classification, subtype discovery, and prediction of outcome in pediatric acute lymphoblastic leukemia by gene expression profiling. Cancer Cell. 2002 Mar;1(2):133-43.
PubMed