Exbio — Research products — Antibodies — Nucleus — Anti-UBN1 Purified

Anti-UBN1 Purified

Regulatory status

RUO

Antigen

UBN1

Clone

UBN1-02

Format

Purified

Reactivity

Human

Application

IP, WB

Variant

0.1 mg

11-565-C100

In stock

198.00 USD

0.025 mg

11-565-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-565-C100

In stock

198.00 USD

0.025 mg

11-565-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The antibody UBN1-02 recognizes N-terminal part of ubinuclein 1 (UBN1), a widely expressed nuclear and adhesion complex protein. Western blotting analysis reveals UBN1 as a 165 kDa band.

Application

IP, WB

Application details

Western blotting: Recommended dilution: 1-5 μg/ml; positive control: HeLa cell line.

Reactivity

Human

Immunogen

Recombinant protein corresponding to amino acids 1-190 of ubinuclein 1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ubinuclein 1, VT4

Antigen description

Ubinuclein 1 (UBN1) is a ubiquitously expressed evolutionarily conserved protein which binds to proliferation-promoting genes that are repressed by formation of senescence-associated heterochromatin foci (SAHF). Ubinuclein 1 associates with various transcription factors and with histone methyltransferase activity, is indispensable for SAHF formation and appears to be a regulator of senescence. Although in most cells ubinuclein is localized to the nucleus, in cells forming tight junctions it is recruited to the cell adhesion complexes, dependently on the cell density.

Entrez Gene ID 29855

UniProt ID Q6PIZ9

Western blotting analysis of ubinuclein in nuclear fraction of HeLa cells. The monoclonal antibody UBN1-01 (lane A, B) detects ubinuclein 1 (165 kDa) and an 180 kDa protein - presumable ubinuclein 2. The antibody UBN1-02 (lane C, D) detects ubinuclein 1. Antibody dilution: A, C - 1 μg/ml; B, D - 5 μg/ml.

General references:

Aho S, Buisson M, Pajunen T, Ryoo YW, Giot JF, Gruffat H, Sergeant A, Uitto J: Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors. J Cell Biol. 2000 Mar 20;148(6):1165-76.
PubMed

Aho S, Lupo J, Coly PA, Sabine A, Castellazzi M, Morand P, Sergeant A, Manet E, Boyer V, Gruffat H: Characterization of the ubinuclein protein as a new member of the nuclear and adhesion complex components (NaCos). Biol Cell. 2008 Sep 30.
PubMed

Banumathy G, Somaiah N, Zhang R, Tang Y, Hoffmann J, Andrake M, Ceulemans H, Schultz D, Marmorstein R, Adams PD: Human UBN1 is an ortholog of yeast Hpc2p and has an essential role in the HIRA/ASF1a chromatin-remodeling pathway in senescent cells. Mol Cell Biol. 2009 Feb;29(3):758-70.
PubMed