Exbio — Research products — Antibodies — Nucleus — Anti-STAT1 Purified

Anti-STAT1 Purified

Regulatory status

RUO

Antigen

STAT1

Clone

SM2

Format

Purified

Reactivity

Human

Application

IP, WB

Variant

0.1 mg

11-405-C100

In stock

198.00 USD

0.025 mg

11-405-C025

In stock

99.00 USD

Variant

0.1 mg

11-405-C100

In stock

198.00 USD

0.025 mg

11-405-C025

In stock

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody SM2 recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Application

IP, WB

Application details

Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.

Reactivity

Human

Immunogen

STAT1 peptide sequence 8-23 (QLDSKFLEQVHQLYD) conjugated to KLH.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CANDF7, IMD31, ISGF3

Antigen description

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Entrez Gene ID 6772

UniProt ID P42224

Western blotting analysis of human STAT1 using mouse monoclonal antibody SM2 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-STAT1 followed by IRDye800-conjugated anti-mouse secondary antibody. STAT1 was detected at approximately 90 kDa.

General references:

Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61.
PubMed

Frank DA: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
PubMed

Bromberg J, Darnell JE Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19(21):2468-73.
PubMed

Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31
PubMed

Product specific references:

Hubackova S, Krejcikova K, Bartek J, Hodny Z: Interleukin 6 signaling regulates promyelocytic leukemia protein gene expression in human normal and cancer cells. J Biol Chem. 2012 Aug 3;287(32):26702-14.
PubMed

Hubackova S, Kucerova A, Michlits G, Kyjacova L, Reinis M, Korolov O, Bartek J, Hodny Z: IFNγ induces oxidative stress, DNA damage and tumor cell senescence via TGFβ/SMAD signaling-dependent induction of Nox4 and suppression of ANT2. Oncogene. 2016 Mar 10;35(10):1236-49.
PubMed