Anti-PCNA Purified

Anti-PCNA Purified
Regulatory status
RUO
Antigen
PCNA
Clone
PC10
Format
Purified
Reactivity
Mouse, Human, Non-human primates, Drosophila, Chicken, Rat
Variant
0.1 mg
11-182-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Mouse, Human, Non-human primates, Drosophila, Chicken, Rat
Immunogen
recombinant rat PCNA
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ATLD2
Antigen description
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.
Entrez Gene ID 5111
UniProt ID P12004
11-182_ICC
Immunocytochemistry staining of PCNA in PHA-stimulated lymphocytes using mouse monoclonal antibody PC10 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200); DNA stained with propidium iodide. Colocalization of green PCNA signal and red DNA signal indicated as yellow.
11-182_IHC
Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-PCNA (PC10), 10 μg/ml. Commercially tested by LifeSpan BioSciences.
11-182 FC
Separation of K562 cells stained using anti-PCNA (PC10) purified antibody (concentration in sample 4 μg/ml, GAM FITC, red) from K562 cells unstained by primary antibody (GAM FITC, blue) in flow cytometry analysis (intracellular staining).

Product specific references:

Dahm K, Hübscher U: Colocalization of human Rad17 and PCNA in late S phase of the cell cycle upon replication block. Oncogene. 2002 Oct 31;21(50):7710-9.
PubMed
Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ: A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19472-7.
PubMed
Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res. 1991 Sep 1;51(17):4570-4.
PubMed
Variant
0.1 mg
11-182-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

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