Exbio — Research products — Antibodies — Nucleus — Anti-PCNA Purified

Anti-PCNA Purified

Regulatory status

RUO

Antigen

PCNA

Clone

PC10

Format

Purified

Reactivity

Mouse, Human, Non-human primates, Drosophila, Chicken, Rat

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

11-182-C100

In stock

143.00 USD

Variant

0.1 mg

11-182-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Mouse, Human, Non-human primates, Drosophila, Chicken, Rat

Immunogen

recombinant rat PCNA

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ATLD2

Antigen description

PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.

Entrez Gene ID 5111

UniProt ID P12004

Separation of K562 cells stained using anti-PCNA (PC10) purified antibody (concentration in sample 4 μg/ml, GAM FITC, red-filled) from K562 cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of PCNA in PHA-stimulated lymphocytes using mouse monoclonal antibody PC10 (diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200); DNA stained with propidium iodide. Colocalization of green PCNA signal and red DNA signal indicated as yellow.

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-PCNA (PC10), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Product specific references:

Dahm K, Hübscher U: Colocalization of human Rad17 and PCNA in late S phase of the cell cycle upon replication block. Oncogene. 2002 Oct 31;21(50):7710-9.
PubMed

Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ: A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19472-7.
PubMed

Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res. 1991 Sep 1;51(17):4570-4.
PubMed