Exbio — Research products — Antibodies — Nucleus — Anti-PCNA Alexa Fluor<sup>®</sup> 647

Anti-PCNA Alexa Fluor^® 647

Regulatory status

RUO

Antigen

PCNA

Clone

PC10

Format

Alexa Fluor^® 647

Reactivity

Mouse, Human, Non-human primates, Drosophila, Chicken, Rat

Application

FC-IC (QC tested), IHC-F, IHC-P, ICC

Excitation laser

red (633 nm)

Variant

100 tests

A6-182-T100

In stock

242.00 USD

Variant

100 tests

A6-182-T100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.

Application

FC-IC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Mouse, Human, Non-human primates, Drosophila, Chicken, Rat

Immunogen

recombinant rat PCNA

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

ATLD2

Antigen description

PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.

Entrez Gene ID 5111

UniProt ID P12004

Separation of K562 cells stained using anti-PCNA (PC10) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml, red-filled) from K562 cells stained using mouse IgG2a isotype control (MOPC-173) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml, same as PCNA Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Dahm K, Hübscher U: Colocalization of human Rad17 and PCNA in late S phase of the cell cycle upon replication block. Oncogene. 2002 Oct 31;21(50):7710-9.
PubMed

Malkas LH, Herbert BS, Abdel-Aziz W, Dobrolecki LE, Liu Y, Agarwal B, Hoelz D, Badve S, Schnaper L, Arnold RJ, Mechref Y, Novotny MV, Loehrer P, Goulet RJ, Hickey RJ: A cancer-associated PCNA expressed in breast cancer has implications as a potential biomarker. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19472-7.
PubMed

Landberg G, Roos G: Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis. Cancer Res. 1991 Sep 1;51(17):4570-4.
PubMed