Exbio — Research products — Antibodies — Nucleus — Anti-PAX5 Purified

Anti-PAX5 Purified

Regulatory status

RUO

Antigen

PAX5

Clone

1H9

Format

Purified

Reactivity

Mouse, Human

Application

ICC (reported), IHC-P (reported), FC-IC (QC tested), IP (reported), WB (reported)

Variant

0.1 mg

11-952-C100

In stock

248.60 USD

Variant

0.1 mg

11-952-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 1H9 recognizes transcription factor PAX5 (intracellular antigen).

Application

ICC (reported), IHC-P (reported), FC-IC (QC tested), IP (reported), WB (reported)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Mouse, Human

Immunogen

GST-tagged recombinant mouse PAX5

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ALL3, BSAP

Antigen description

PAX5 is a transcription factor expressed in hematopoietic cells of B cell line, and partially also in the nervous system. It is important for B cell development and differentiation, but its expression must finally be be suppressed to enable plasma cell maturation. PAX5 thus affects the balance between B cell proliferation and antibody production. Its overexpression is associated with many lymphomas.

Entrez Gene ID 5079

UniProt ID Q02548

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-PAX5 (1H9) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Separation of human PAX5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-PAX5 (1H9) purified antibody (concentration in sample 1.67 μg/ml, GAM APC).

Product specific references:

Liu GJ, Cimmino L, Jude JG, Hu Y, Witkowski MT, McKenzie MD, Kartal-Kaess M, Best SA, Tuohey L, Liao Y, Shi W, Mullighan CG, Farrar MA, Nutt SL, Smyth GK, Zuber J, Dickins RA: Pax5 loss imposes a reversible differentiation block in B-progenitor acute lymphoblastic leukemia. Genes Dev. 2014 Jun 15;28(12):1337-50.
PubMed

Villanueva-Hernández S, Razavi MA, van Dongen KA, Stadler M, de Luca K, Beyersdorf N, Saalmüller A, Gerner W, Mair KH: Co-expression of the B-cell key transcription factors Blimp-1 and IRF4 identifies plasma cells in the pig. Front Immunol. 2022 Apr 8:13:854257.
PubMed