Exbio — Research products — Antibodies — Nucleus — Anti-PAX5 Alexa Fluor<sup>®</sup> 647

Anti-PAX5 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

PAX5

Clone

1H9

Format

Alexa Fluor^® 647

Reactivity

Mouse, Human

Application

FC-IC (QC tested), IHC-P, ICC

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-952-C100

In stock

248.60 USD

Variant

0.1 mg

A6-952-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 1H9 recognizes transcription factor PAX5 (intracellular antigen).

Application

FC-IC (QC tested), IHC-P, ICC

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Mouse, Human

Immunogen

GST-tagged recombinant mouse PAX5

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

ALL3, BSAP

Antigen description

PAX5 is a transcription factor expressed in hematopoietic cells of B cell line, and partially also in the nervous system. It is important for B cell development and differentiation, but its expression must finally be be suppressed to enable plasma cell maturation. PAX5 thus affects the balance between B cell proliferation and antibody production. Its overexpression is associated with many lymphomas.

Entrez Gene ID 5079

UniProt ID Q02548

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-PAX5 (1H9) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD19 (LT19) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and intracellular staining using anti-PAX5 (1H9) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).

Separation of human PAX5 positive CD19 positive B cells (red-filled) from PAX5 negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-PAX5 (1H9) Alexa Fluor® 647 antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Liu GJ, Cimmino L, Jude JG, Hu Y, Witkowski MT, McKenzie MD, Kartal-Kaess M, Best SA, Tuohey L, Liao Y, Shi W, Mullighan CG, Farrar MA, Nutt SL, Smyth GK, Zuber J, Dickins RA: Pax5 loss imposes a reversible differentiation block in B-progenitor acute lymphoblastic leukemia. Genes Dev. 2014 Jun 15;28(12):1337-50.
PubMed

Villanueva-Hernández S, Razavi MA, van Dongen KA, Stadler M, de Luca K, Beyersdorf N, Saalmüller A, Gerner W, Mair KH: Co-expression of the B-cell key transcription factors Blimp-1 and IRF4 identifies plasma cells in the pig. Front Immunol. 2022 Apr 8:13:854257.
PubMed