Exbio — Research products — Antibodies — Nucleus — Anti-NR2F6 Purified

Anti-NR2F6 Purified

Regulatory status

RUO

Antigen

NR2F6

Clone

EM-51

Format

Purified

Reactivity

Human

Application

FC(IC), WB

Variant

0.1 mg

11-719-C100

In stock

198.00 USD

0.025 mg

11-719-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-719-C100

In stock

198.00 USD

0.025 mg

11-719-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody EM-51 recognizes NR2F6, a transcriptional repressor (intracellular antigen) expressed mainly in the heart, placenta, liver, skeletal muscle, kidney and pancreas, but also e.g. in T cell subpopulations.

Application

FC(IC), WB

Application details

Western blotting: Positive control: HEK293T/17/NR2F6/Ha-tag transfectants, negative control: HEK293T/17 cells.
Flow cytometry: Recommended dilution: 3-9 µg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Recombinant human NR2F6 protein expressed in E. coli

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

EAR2, EAR-2, ERBAL2

Antigen description

NR2F6 (nuclear receptor subfamily 2 group F member 6), also known as EAR2 or ERBAL2, is a transcription factor involved in modulation of hormonal responses. NR2F6 represses e.g. transcription of the oxytocin-neurophysin gene, renin gene, lutropin-choriogonadotropic hormone receptor gene, and the thyroid hormone receptor gene. In the immune system, NR2F6 affects IL-17 expression in the Th-17 cells, thus also the balance between immunological tolerance and autoimmunity.

Entrez Gene ID 2063

UniProt ID P10588

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-NR2F6 (EM-51) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Separation of human monocytes stained using anti-NR2F6 (EM-51) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).