Anti-Notch1 Purified

Anti-Notch1 Purified
Regulatory status
RUO
Antigen
Notch1
Clone
mN1A
Format
Purified
Reactivity
Mouse, Human
Variant
0.1 mg
11-651-C100
In stock
130.00 USD

0.025 mg
11-651-C025
In stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Mouse, Human
Negative species
Rat
Immunogen
GST fusion protein containing cdc10-NCR region of mouse Notch1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AOS5, TAN1, hN1, AOVD1
Antigen description
Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
Entrez Gene ID 4851
UniProt ID P46531
11-651 FC
Separation of Jurkat cells stained using antianti-Notch1 (mN1A) purified antibody (concentration in sample 16 μg/ml, GAM APC, red) from Jurkat cells unstained by primary antibody (GAM APC, black) in flow cytometry analysis (surface staining).

Product specific references:

Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48.
PubMed
Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57.
PubMed
Variant
0.1 mg
11-651-C100
In stock
130.00 USD

0.025 mg
11-651-C025
In stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD

Related products