Exbio — Research products — Antibodies — Nucleus — Anti-Notch1 PE

Anti-Notch1 PE

Regulatory status

RUO

Antigen

Notch1

Clone

mN1A

Format

Reactivity

Mouse, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-651-C100

In stock

180.00 USD

0.025 mg

1P-651-C025

Delivery 1 week

90.00 USD

Variant

0.1 mg

1P-651-C100

In stock

180.00 USD

0.025 mg

1P-651-C025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Mouse, Human

Negative species

Rat

Immunogen

GST fusion protein containing cdc10-NCR region of mouse Notch1

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

AOS5, TAN1, hN1, AOVD1

Antigen description

Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.

Entrez Gene ID 4851

UniProt ID P46531

Flow cytometry multicolor surface staining pattern of human PHA stimulated CD3 positive lymphocytes using anti-human CD25 (MEM-181) APC antibody (10 μl reagent / 100 μl of sample) and intracellular staining of human lymphocytes using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml).

Separation of CD3 positive CD25 positive cells stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml, red-filled) from CD3 positive CD25 positive cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as Notch1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of PHA stimulated human peripheral whole blood .

Flow cytometry intracellular staining patterns of PHA stimulated human peripheral whole blood stained using anti-Notch1 (mN1A) PE antibody (concentration in sample 3 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as Notch1 PE concentration, right).

Product specific references:

Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice. J Clin Invest. 2010 Jul 1;120(7):2537-48.
PubMed

Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J Cell Biol. 2007 May 21;177(4):647-57.
PubMed