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Exbio
—
Research products
—
Antibodies
—
Nucleus
—
Anti-Ki-67 Purified
Anti-Ki-67 Purified
Regulatory status
RUO
Antigen
Ki-67
Clone
Ki-67
Format
Purified
Reactivity
Cow, Human
Application
IHC, WB, FC (QC tested), ICC
Variant
0.1 mg
11-155-C100
In stock
143.00 USD
0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-155-C100
In stock
143.00 USD
0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Application
IHC, WB, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.
Reactivity
Cow, Human
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
KIA, MIB, Marker of proliferation Ki67, MKI67
Antigen description
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Entrez Gene ID
4288
UniProt ID
P46013
Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).
Separation of human Ki-67 positive cells (red-filled) from Ki-67 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
Flow cytometry intracellular staining pattern of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
Product specific references:
Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22.
PubMed
Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21.
PubMed
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5.
PubMed
Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206.
PubMed
Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20.
PubMed
Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov;185(1):50-9.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
11-155-C100
In stock
143.00 USD
0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-155-C100
In stock
143.00 USD
0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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