Anti-Ki-67 Purified

Anti-Ki-67 Purified
Regulatory status
RUO
Antigen
Ki-67
Clone
Ki-67
Format
Purified
Reactivity
Cow, Human
Variant
0.1 mg
11-155-C100
In stock
143.00 USD

0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-155-C100
In stock
143.00 USD

0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Application details
Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.
Reactivity
Cow, Human
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
KIA, MIB, Marker of proliferation Ki67, MKI67
Antigen description
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Entrez Gene ID 4288
UniProt ID P46013
11-155_ICC
Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).
11-155_FC_Histogram
Separation of human Ki-67 positive cells (red-filled) from Ki-67 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.
11-155_FC_Profil Značení
Flow cytometry intracellular staining pattern of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Product specific references:

Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22.
PubMed
Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21.
PubMed
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5.
PubMed
Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206.
PubMed
Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20.
PubMed
Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov;185(1):50-9.
PubMed
Variant
0.1 mg
11-155-C100
In stock
143.00 USD

0.025 mg
11-155-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-155-C100
In stock
143.00 USD

0.025 mg
11-155-C025
Delivery 1 week
71.50 USD

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