Exbio — Research products — Antibodies — Nucleus — Anti-Ki-67 Purified

Anti-Ki-67 Purified

Regulatory status

RUO

Antigen

Ki-67

Clone

Ki-67

Format

Purified

Reactivity

Cow, Human

Application

IHC, WB, FC (QC tested), ICC

Variant

0.1 mg

11-155-C100

In stock

143.00 USD

0.025 mg

11-155-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-155-C100

In stock

143.00 USD

0.025 mg

11-155-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.

Application

IHC, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.
Immunocytochemistry: Paraformaldehyde fixation; recommended antibody concentration 1 μg/ml.

Reactivity

Cow, Human

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

KIA, MIB, Marker of proliferation Ki67, MKI67

Antigen description

Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.

Entrez Gene ID 4288

UniProt ID P46013

Immunocytochemistry detection of Ki-67 in U2OS cell line (human osteosarcoma) using monoclonal antibody Ki-67 (green). Cell nuclei stained with DAPI (blue).

Separation of human Ki-67 positive cells (red-filled) from Ki-67 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Flow cytometry intracellular staining pattern of human PHA stimulated peripheral whole blood stained using anti-human Ki-67 (Ki-67) purified antibody (concentration in sample 0.6 μg/ml) GAM APC.

Product specific references:

Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22.
PubMed

Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21.
PubMed

Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5.
PubMed

Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206.
PubMed

Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20.
PubMed

Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov;185(1):50-9.
PubMed