Exbio — Research products — Antibodies — Nucleus — Anti-Ki-67 Alexa Fluor<sup>®</sup> 488

Anti-Ki-67 Alexa Fluor® 488

Anti-Ki-67 Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
Ki-67
Clone
Ki-67
Format
Alexa Fluor® 488
Reactivity
Cow, Human
Application
WB, FC (QC tested), ICC
Excitation laser
blue (488 nm)
Variant
100 tests
A4-155-T100
In stock
220.00 USD
25 tests
A4-155-T025
Delivery 1 week
110.00 USD
Variant
100 tests
A4-155-T100
In stock
220.00 USD
25 tests
A4-155-T025
Delivery 1 week
110.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Application
WB, FC (QC tested), ICC
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Cow, Human
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
KIA, MIB, Marker of proliferation Ki67, MKI67
Antigen description
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Entrez Gene ID 4288
UniProt ID P46013
A4-155 FC
Separation of Jurkat cells stained using anti-Ki-67(Ki-67) Alexa Fluor® 488 antibody (concentration in sample 9 μg/ml, red) from unstained Jurkat cells (black) in flow cytometry analysis (intracellular staining).

Product specific references:

Schlüter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J: The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol. 1993 Nov;123(3):513-22.
PubMed
Duchrow M, Schlüter C, Key G, Kubbutat MH, Wohlenberg C, Flad HD, Gerdes J: Cell proliferation-associated nuclear antigen defined by antibody Ki-67: a new kind of cell cycle-maintaining proteins. Arch Immunol Ther Exp (Warsz). 1995;43(2):117-21.
PubMed
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H: Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984 Oct;133(4):1710-5.
PubMed
Gerdes J: Ki-67 and other proliferation markers useful for immunohistological diagnostic and prognostic evaluations in human malignancies. Semin Cancer Biol. 1990 Jun;1(3):199-206.
PubMed
Gerdes J, Schwab U, Lemke H, Stein H: Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 1983 Jan 15;31(1):13-20.
PubMed
Bading H, Rauterberg EW, Moelling K: Distribution of c-myc, c-myb, and Ki-67 antigens in interphase and mitotic human cells evidenced by immunofluorescence staining technique. Exp Cell Res. 1989 Nov;185(1):50-9.
PubMed
Variant
100 tests
A4-155-T100
In stock
220.00 USD
25 tests
A4-155-T025
Delivery 1 week
110.00 USD
Variant
100 tests
A4-155-T100
In stock
220.00 USD
25 tests
A4-155-T025
Delivery 1 week
110.00 USD
Contact distributor
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