Anti-Ikaros PE

Anti-Ikaros PE
Regulatory status
RUO
Antigen
Ikaros
Clone
4E9
Format
PE
Reactivity
Mouse, Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-159-T100
In stock
220.00 USD

25 tests
1P-159-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Mouse, Human
Immunogen
Recombinant human Ikaros (C-terminal part)
Other names
IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452; IKAROS family zinc finger 1; Lymphoid transcription factor LyF-1
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-159 FC
Flow cytometry analysis (intracellular staining) of Ikaros in human peripheral blood leukocytes with anti-Ikaros (4E9) PE (red), compared with blank (blue).

Product specific references:

López-Nieva P, Santos J, Fernández-Piqueras J: Defective expression of Notch1 and Notch2 in connection to alterations of c-Myc and Ikaros in gamma-radiation-induced mouse thymic lymphomas. Carcinogenesis. 2004 Jul;25(7):1299-304.
PubMed
Loeper S, Asa SL, Ezzat S: Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer Res. 2008 May 15;68(10):3715-23.
PubMed
Kathrein KL, Lorenz R, Innes AM, Griffiths E, Winandy S: Ikaros induces quiescence and T-cell differentiation in a leukemia cell line. Mol Cell Biol. 2005 Mar;25(5):1645-54.
PubMed
Schwickert TA, Tagoh H, Gültekin S, Dakic A, Axelsson E, Minnich M, Ebert A, Werner B, Roth M, Cimmino L, Dickins RA, Zuber J, Jaritz M, Busslinger M: Stage-specific control of early B cell development by the transcription factor Ikaros. Nat Immunol. 2014 Mar;15(3):283-93.
PubMed
Elliott J, Jolicoeur C, Ramamurthy V, Cayouette M: Ikaros confers early temporal competence to mouse retinal progenitor cells. Neuron. 2008 Oct 9;60(1):26-39.
PubMed
Koipally J, Heller EJ, Seavitt JR, Georgopoulos K: Unconventional potentiation of gene expression by Ikaros. J Biol Chem. 2002 Apr 12;277(15):13007-15.
PubMed
Variant
100 tests
1P-159-T100
In stock
220.00 USD

25 tests
1P-159-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD