Exbio — Research products — Antibodies — Nucleus — Anti-Ikaros Alexa Fluor<sup>®</sup> 647

Anti-Ikaros Alexa Fluor^® 647

Regulatory status

RUO

Antigen

Ikaros

Clone

4E9

Format

Alexa Fluor^® 647

Reactivity

Mouse, Human

Application

FC-IC (QC tested)

Excitation laser

red (633 nm)

test1 True

Variant

100 tests

A6-159-T100

In stock

248.60 USD

Variant

100 tests

A6-159-T100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 4E9 recognizes Ikaros, a transcription factor (intracellular antigen) expressed broadly in hematopoietic progenitors and serving as a key regulator of lymphopoiesis.

Application

FC-IC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Mouse, Human

Immunogen

Recombinant human Ikaros (C-terminal part)

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

IKZF1; IK1; LYF1; LyF-1; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452; IKAROS family zinc finger 1; Lymphoid transcription factor LyF-1

Antigen description

Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Helios. Ikaros forms homo- and heterodimers with these proteins and functions predominantly in early hematopoietic development. Expression of Ikaros, Aiolos and Helios is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Disruption of Ikaros leads to T and B cell leukemias.

Entrez Gene ID 10320

UniProt ID Q13422

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Ikaros (4E9) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml).

Separation of human lymphocytes (red-filled) from Ikaros negative blood debris (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-Ikaros (4E9) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml).

Product specific references:

López-Nieva P, Santos J, Fernández-Piqueras J: Defective expression of Notch1 and Notch2 in connection to alterations of c-Myc and Ikaros in gamma-radiation-induced mouse thymic lymphomas. Carcinogenesis. 2004 Jul;25(7):1299-304.
PubMed

Loeper S, Asa SL, Ezzat S: Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer Res. 2008 May 15;68(10):3715-23.
PubMed

Kathrein KL, Lorenz R, Innes AM, Griffiths E, Winandy S: Ikaros induces quiescence and T-cell differentiation in a leukemia cell line. Mol Cell Biol. 2005 Mar;25(5):1645-54.
PubMed

Schwickert TA, Tagoh H, Gültekin S, Dakic A, Axelsson E, Minnich M, Ebert A, Werner B, Roth M, Cimmino L, Dickins RA, Zuber J, Jaritz M, Busslinger M: Stage-specific control of early B cell development by the transcription factor Ikaros. Nat Immunol. 2014 Mar;15(3):283-93.
PubMed

Elliott J, Jolicoeur C, Ramamurthy V, Cayouette M: Ikaros confers early temporal competence to mouse retinal progenitor cells. Neuron. 2008 Oct 9;60(1):26-39.
PubMed

Koipally J, Heller EJ, Seavitt JR, Georgopoulos K: Unconventional potentiation of gene expression by Ikaros. J Biol Chem. 2002 Apr 12;277(15):13007-15.
PubMed