Exbio — Research products — Antibodies — Nucleus — Anti-Hu TdT Purified

Anti-Hu TdT Purified

Regulatory status

RUO

Antigen

TdT

Clone

41A

Format

Purified

Reactivity

Human

Application

ELISA, WB, FC (QC tested), IHC(P)

Variant

0.1 mg

11-868-C100

In stock

198.00 USD

Variant

0.1 mg

11-868-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase; an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.

Application

ELISA, WB, FC (QC tested), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

His-TdT recombinant protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Terminal transferase, DNTT, Terminal addition enzyme

Antigen description

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Entrez Gene ID 1791

UniProt ID P04053

Separation of REH cells stained anti-human TdT (41A) purified antibody (concentration in sample 0,9 μg/ml, GAM APC, red-filled) from REH cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 7;24(3):376-389.e8.
PubMed