Exbio — Research products — Antibodies — Nucleus — Anti-Hu TdT FITC

Anti-Hu TdT FITC

Regulatory status

RUO

Antigen

TdT

Clone

41A

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-868-T100

In stock

198.00 USD

Variant

100 tests

1F-868-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase; an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for intracellular analysis of human blood cells after XPerm (fixation and permeabilization reagent, ED7397) permeabilization of cell membrane and using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

His-TdT recombinant protein

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Terminal transferase, DNTT, Terminal addition enzyme

Antigen description

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Entrez Gene ID 1791

UniProt ID P04053

Flow cytometry intacellular staining (Excellyse XPerm, cat. no. ED7397) pattern of human peripheral whole blood spiked with REH cellular suspension stained using anti-human TdT (41A) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood spiked with REH cells).

1F-868_FC_ALL-Profil Značení-ALL-CD45+TdT

Flow cytometry staining patterns of human peripheral whole blood of acute lymphoblastic leukemia patient.
On left there is surface staining pattern of the sample using anti-human CD45 (MEM-28) PerCP antibody (10 μl reagent / 100 μl of peripheral whole blood).
On right there is intracellular staining (Excellyse XPerm, cat. no. ED7397) pattern of the sample using anti-human TdT (41A) FITC antibody.
Red are TdT positive events - lymphoblastic cells.

Separation of REH cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining, Excellyse XPerm, cat. no. ED7397) of human peripheral whole blood spiked with REH cellular suspension stained using anti-human TdT (41A) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood spiked with REH cells).

Product specific references:

Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 7;24(3):376-389.e8.
PubMed