Clinical products
Premium antibodies (GMP)
Intracellular staining
Surface staining
Hematology
Kits
Lysing solutions
Multicolor cocktails
Single color antibodies
Immunodeficiency
Kits
Lysing solutions
Mitogens
Multicolor cocktails
Single color antibodies
Reproductive immunology
Kits
Lysing solutions
Multicolor cocktails
New products
Allergy
Kits
Recombinant allergens
Allergen extracts
Other allergens
Research products
Antibodies
CD and related antigens
Blood group antigens
Multicolor cocktails
Serum, plasma, and secreted proteins
Cytoskeleton and ECM
Isotype controls
MHC antigens
Nucleus
Other intracellular antigens
Small organic molecules
Tags and labels
Microbiology and virology
Accessory reagents
Annexin V
DNA-binding probes
Lineage cocktail
Lysing solutions
Recombinant proteins
Streptavidin
Recombinant allergens
Animal
Food
House Dust Mite
Mold
Pollen
Venom
Kits
New products
Dry Reagents
About exbio
Company
OEM and Commercial Supplies
Events and Conferences
Blog
Inquiries
Technical resources
International distributors
Contact
Jobs
IVDR
E-mail
*
Password
*
Remember me
Forgotten password
Log in
Register
E-mail
Send request
Log in
Exbio
—
Research products
—
Antibodies
—
Nucleus
—
Anti-Hu TdT Alexa Fluor<sup>®</sup> 647
Anti-Hu TdT Alexa Fluor
®
647
Regulatory status
RUO
Antigen
TdT
Clone
41A
Format
Alexa Fluor
®
647
Reactivity
Human
Application
WB, FC (QC tested), IHC-P
Excitation laser
red (633 nm)
Variant
100 tests
A6-868-T100
In stock
242.00 USD
Variant
100 tests
A6-868-T100
In stock
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase; an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.
Application
WB, FC (QC tested), IHC-P
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Human
Immunogen
His-TdT recombinant protein
Preparation
Purified antibody is conjugated with Alexa Fluor
®
647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
Terminal transferase, DNTT, Terminal addition enzyme
Antigen description
Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.
Entrez Gene ID
1791
UniProt ID
P04053
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human TdT (41A) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).
Separation of REH cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with REH cells and stained using anti-human TdT (41A) Alexa Fluor® 647 antibody (4 μl reagent / 100 μl of peripheral whole blood).
Product specific references:
Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 7;24(3):376-389.e8.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
A6-868-T100
In stock
242.00 USD
Variant
100 tests
A6-868-T100
In stock
242.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
A6-724-C100
Mouse IgG2a Isotype Control Alexa Fluor
®
647
-134%
Clone
MOPC-173
Reg. status
RUO
Application
FC, FC-IC, WB, IHC-F, IHC-P, ICC
0.1 mg
231.00 USD
Continue to checkout
×
Zavřít