Anti-Hu STAT1 (pS727) Purified

Anti-Hu STAT1 (pS727) Purified
Regulatory status
RUO
Antigen
STAT1 (pS727)
Clone
PSM1
Format
Purified
Reactivity
Human
Application
IP, WB
Variant
0.1 mg
11-372-C100
In stock
0.00 USD

0.025 mg
11-372-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

0.1 mg
11-261-C100
In stock
0.00 USD
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727 (intracellular antigen).
Application
IP, WB
Reactivity
Human
Immunogen
STAT1 peptide sequence 721-733 (Ser727 phosphorylated).
Other names
CANDF7, IMD31, ISGF3
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-372 WB
Western blotting analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-gamma treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2; cat. no. 11-405-C100) Lane 2: original whole cell lysate

General references:

Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61.
PubMed
Frank DA: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
PubMed
Bromberg J, Darnell JE Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19(21):2468-73.
PubMed
Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31
PubMed

Product specific references:

Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M: Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation. Int J Mol Med. 2003 Sep;12(3):335-40.
PubMed
Boudny V, Kocak I, Lauerova L, Kovarik J: Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients. Folia Biol (Praha). 2003;49(4):142-6.
PubMed
Variant
0.1 mg
11-372-C100
In stock
0.00 USD

0.025 mg
11-372-C025
Out of stock
0.00 USD
Variant
0.1 mg
11-261-C100
In stock
0.00 USD

0.1 mg
11-261-C100
In stock
0.00 USD