Exbio — Research products — Antibodies — Nucleus — Anti-Hu STAT1 (pS727) Purified

Anti-Hu STAT1 (pS727) Purified

Regulatory status

RUO

Antigen

STAT1 (pS727)

Clone

PSM1

Format

Purified

Reactivity

Human

Application

IP, WB

Variant

0.1 mg

11-372-C100

In stock

198.00 USD

0.025 mg

11-372-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-372-C100

In stock

198.00 USD

0.025 mg

11-372-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727 (intracellular antigen).

Application

IP, WB

Reactivity

Human

Immunogen

STAT1 peptide sequence 721-733 (Ser727 phosphorylated).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CANDF7, IMD31, ISGF3

Antigen description

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Entrez Gene ID 6772

UniProt ID P42224

Western blotting analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-gamma treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2; cat. no. 11-405-C100) Lane 2: original whole cell lysate

General references:

Kovarik P, Stoiber D, Eyers PA, Menghini R, Neininger A, Gaestel M, Cohen P, Decker T: Stress-induced phosphorylation of STAT1 at Ser727 requires p38 mitogen-activated protein kinase whereas IFN-gamma uses a different signaling pathway. Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):13956-61.
PubMed

Frank DA: STAT signaling in the pathogenesis and treatment of cancer. Mol Med. 1999 Jul;5(7):432-56.
PubMed

Bromberg J, Darnell JE Jr: The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000 May 15;19(21):2468-73.
PubMed

Soond SM, Townsend PA, Barry SP, Knight RA, Latchman DS, Stephanou A: ERK and the E3 ubiquitin ligase beta TRCP targets STAT1 for degradation. J Biol Chem. 2008 Mar 31
PubMed

Product specific references:

Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M: Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation. Int J Mol Med. 2003 Sep;12(3):335-40.
PubMed

Boudny V, Kocak I, Lauerova L, Kovarik J: Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients. Folia Biol (Praha). 2003;49(4):142-6.
PubMed