Anti-HDAC6 Purified

Anti-HDAC6 Purified
Regulatory status
RUO
Antigen
HDAC6
Clone
159
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-841-C100
In stock
154.00 USD
Variant
0.1 mg
11-841-C100
In stock
154.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 159 recognizes SE domain of human histone deacetylase 6 (HDAC6; an intracellular antigen), amino acids 971-981.
Application details
Western blotting: Recommended dilution: 1-5 μg/ml.
Immunocytochemistry: Recommended dilution: 5-10 μg/ml.
Reactivity
Human
Negative species
Mouse, Danio
Immunogen
human HDAC6
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Histone deacetylase 6
Antigen description
Histone deacetylase 6 (HDAC6), like other histone deacetylases, affects gene expression by regulation of chromatin remodeling. HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other. Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. Deregulation of HDAC6 expression and activity is associated with many diseases.
Entrez Gene ID 10013
UniProt ID Q9NSW6
11-841_FC_Histogram
Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 μg/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
11-841_ELISA
ELISA analysis of human HDAC6 by mouse monoclonal antibody 159. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.
11-841_ICC
Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 159, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

General references:

Seidel C, Schnekenburger M, Dicato M, Diederich M: Histone deacetylase 6 in health and disease. Epigenomics 2015; 7(1): 103-118.
PubMed
Variant
0.1 mg
11-841-C100
In stock
154.00 USD
Variant
0.1 mg
11-841-C100
In stock
154.00 USD

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