Exbio — Research products — Antibodies — Nucleus — Anti-HDAC6 Purified

Anti-HDAC6 Purified

Regulatory status

RUO

Antigen

HDAC6

Clone

236

Format

Purified

Reactivity

Human

Application

ELISA, IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-843-C100

In stock

140.00 USD

Variant

0.1 mg

11-843-C100

In stock

140.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 236 recognizes D1 domain of human histone deacetylase 6 (HDAC6; intracellular antigen), amino acids 71-91.

Application

ELISA, IP, WB, FC (QC tested), ICC

Application details

Western blotting: Recommended dilution: 1-5 μg/ml.
Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

Reactivity

Human

Negative species

Mouse, Danio

Immunogen

human HDAC6

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Histone deacetylase 6

Antigen description

Histone deacetylase 6 (HDAC6), like other histone deacetylases, affects gene expression by regulation of chromatin remodeling. HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other. Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. Deregulation of HDAC6 expression and activity is associated with many diseases.

Entrez Gene ID 10013

UniProt ID Q9NSW6

Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody 236, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).

ELISA analysis of human HDAC6 by mouse monoclonal antibody 236. MaxiSorp plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.

Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and 236 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.

Western blotting analysis of human HDAC6 by mouse monoclonal antibody 236 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 µg/ml), followed by HRP-conjugated anti-mouse secondary antibody.