Exbio — Research products — Antibodies — Nucleus — Anti-HDAC6 Alexa Fluor<sup>®</sup> 488

Anti-HDAC6 Alexa Fluor^® 488

Regulatory status

RUO

Antigen

HDAC6

Clone

236

Format

Alexa Fluor^® 488

Reactivity

Human

Application

WB, FC (QC tested), ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-843-C100

In stock

242.00 USD

Variant

0.1 mg

A4-843-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody 236 recognizes D1 domain of human histone deacetylase 6 (HDAC6; intracellular antigen), amino acids 71-91.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Human

Negative species

Mouse, Danio

Immunogen

human HDAC6

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Histone deacetylase 6

Antigen description

Histone deacetylase 6 (HDAC6), like other histone deacetylases, affects gene expression by regulation of chromatin remodeling. HDAC6 contains an internal duplication of two catalytic domains which appear to function independently of each other. Besides histones, HDAC6 deacetylates also other substrates including alpha tubulin and HSP90 alpha, and is involved in protein trafficking and degradation, as well as in affecting of cell shape and migration. Deregulation of HDAC6 expression and activity is associated with many diseases.

Entrez Gene ID 10013

UniProt ID Q9NSW6

Separation of Jurkat cells stained using anti-HDAC6 (236) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml, red-filled) from Jurkat cells stained using mouse isotype control Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml, same as HDAC6 Alexa Fluor® 488 concentration, black-dashed) in flow cytometry analysis (intracellular staining) of Jurkat cell suspension.