Anti-Galectin-3 Purified

Anti-Galectin-3 Purified
Regulatory status
RUO
Antigen
Galectin-3
Clone
M3/38
Format
Purified
Reactivity
Human, Mouse
Variant
0.1 mg
11-876-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody M3/38 recognizes an epitope within amino acids 40-100 in N-terminal domain of murine galectin 3, a 35 kDa lectin expressed both intracellularly, and after its secretion also on the cell surface.
Application details
Flow cytometry: Recommended dilution: 2-6 μg/ml. Extracellular and intracellular staining.
Reactivity
Human, Mouse
Immunogen
murine galectin-3
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Mac-2, GBP, Gal-3, L-34
Antigen description
Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.
Entrez Gene ID 16854
UniProt ID P16110
11-876_FC_Histogram
Separation of SK-MEL-30 cells stained using anti-Galectin-3 (M3/38) purified antibody (concentration in sample 2,2 μg/ml, DAR APC, red-filled) from SK-MEL-30 cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of SK-MEL-30 cell suspension.

Product specific references:

Fogel S, Guittaut M, Legrand A, Monsigny M, Hébert E: The tat protein of HIV-1 induces galectin-3 expression. Glycobiology. 1999 Apr;9(4):383-7.
PubMed
Sundqvist M, Welin A, Elmwall J, Osla V, Nilsson UJ, Leffler H, Bylund J, Karlsson A: Galectin-3 type-C self-association on neutrophil surfaces; The carbohydrate recognition domain regulates cell function. J Leukoc Biol. 2018 Feb;103(2):341-353.
PubMed
Nachtigal M, Al-Assaad Z, Mayer EP, Kim K, Monsigny M: Galectin-3 expression in human atherosclerotic lesions. Am J Pathol. 1998 May;152(5):1199-208.
PubMed
Variant
0.1 mg
11-876-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD

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