Exbio — Research products — Antibodies — Nucleus — Anti-Galectin-3 Alexa Fluor<sup>®</sup> 488

Anti-Galectin-3 Alexa Fluor^® 488

Regulatory status

RUO

Antigen

Galectin-3

Clone

M3/38

Format

Alexa Fluor^® 488

Reactivity

Human, Mouse

Application

FC, FC-IC (QC tested), IHC-P, ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-876-C100

In stock

242.00 USD

Variant

0.1 mg

A4-876-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody M3/38 recognizes an epitope within amino acids 40-100 in N-terminal domain of murine galectin 3, a 35 kDa lectin expressed both intracellularly, and after its secretion also on the cell surface.

Application

FC, FC-IC (QC tested), IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human, Mouse

Immunogen

murine galectin-3

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Mac-2, GBP, Gal-3, L-34

Antigen description

Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.

Entrez Gene ID 16854

UniProt ID P16110

Separation of SK-MEL-30 cells stained using anti-human Galectin-3 (M3/38) Alexa Fluor® 488 antibody (concentration in sample 5 μg/m, red-filled) from unstained SK-MEL-30 cells (black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Fogel S, Guittaut M, Legrand A, Monsigny M, Hébert E: The tat protein of HIV-1 induces galectin-3 expression. Glycobiology. 1999 Apr;9(4):383-7.
PubMed

Sundqvist M, Welin A, Elmwall J, Osla V, Nilsson UJ, Leffler H, Bylund J, Karlsson A: Galectin-3 type-C self-association on neutrophil surfaces; The carbohydrate recognition domain regulates cell function. J Leukoc Biol. 2018 Feb;103(2):341-353.
PubMed

Nachtigal M, Al-Assaad Z, Mayer EP, Kim K, Monsigny M: Galectin-3 expression in human atherosclerotic lesions. Am J Pathol. 1998 May;152(5):1199-208.
PubMed