Anti-FoxP3 Purified

Anti-FoxP3 Purified
Regulatory status
RUO
Antigen
FoxP3
Clone
3G3
Format
Purified
Reactivity
Human, Mouse
Variant
0.1 mg
11-601-C100
In stock
210.00 USD

0.025 mg
11-601-C025
Delivery 1 week
105.00 USD
Variant
0.1 mg
11-261-C100
In stock
210.00 USD

0.1 mg
11-261-C100
In stock
105.00 USD
Product details
Description
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.
Application details
Western blotting: Recommended dilution: 2 μg/ml.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Reactivity
Human, Mouse
Immunogen
Full-length His-tagged recombinant murine FoxP3
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER
Antigen description
FoxP3 (forkhead box protein 3), a highly conserved forkhead/winged-helix transcription factor, plays a crucial role in maintaining immune homeostasis by governing the development and function of regulatory T cells. It is constitutively expressed at high level in CD25+ CD4+ Treg cells and at low level in a CD25- CD4+ Treg cell subset. Defects in gene encoding FoxP3 protein cause the scurfy phenotype in mice, and in human the IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome.
Entrez Gene ID 50943
UniProt ID Q9BZS1

General references:

Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301(1):71-81.
PubMed
Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114(18):3727-35.
PubMed
Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31(3):401-11.
PubMed
Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21(6):612-8.
PubMed
Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7.
PubMed

Product specific references:

Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75(12):1040-50.
PubMed
Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64.
PubMed
Variant
0.1 mg
11-601-C100
In stock
210.00 USD

0.025 mg
11-601-C025
Delivery 1 week
105.00 USD
Variant
0.1 mg
11-261-C100
In stock
210.00 USD

0.1 mg
11-261-C100
In stock
105.00 USD

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