Anti-FoxP3 Alexa Fluor® 647

Anti-FoxP3 Alexa Fluor® 647
Regulatory status
RUO
Antigen
FoxP3
Clone
3G3
Format
Alexa Fluor® 647
Reactivity
Human, Mouse
Application
WB, FC (QC tested)
Excitation laser
red (633 nm)
Variant
0.1 mg
A6-601-C100
In stock
310.00 USD

0.025 mg
A6-601-C025
Out of stock
155.00 USD
Variant
0.1 mg
11-261-C100
In stock
310.00 USD

0.1 mg
11-261-C100
In stock
155.00 USD
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 3G3 recognizes N-terminal region of FoxP3, a 47-55 kDa transcription factor (intracellular antigen), which is the master regulator in the development and function of regulatory T cells.
Application
WB, FC (QC tested)
Reactivity
Human, Mouse
Immunogen
Full-length His-tagged recombinant murine FoxP3
Other names
Scurfin, JM2, AIID, IPEX, PIDX, XPID, DIETER
Concentration
0.5 mg/ml
Preparation
The purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A6-601_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml).
A6-601_FC_Dot-plot
Flow cytometry multicolor surface staining of human CD4 positive lymphocytes using anti-human CD25 (MEM-181) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human CD4 positive lymphocytes using anti-human FoxP3 (3G3) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml).
A6-601_FC_Histogram
Separation of FoxP3 positive CD25 positive CD4 positive lymphocytes (red-filled) from FoxP3 negative CD25 negative CD4 positive lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor® 647 antibody (concentration in sample 3 μg/ml).

General references:

Kuhn A, Beissert S, Krammer PH: CD4(+)CD25 (+) regulatory T cells in human lupus erythematosus. Arch Dermatol Res. 2009 Jan;301(1):71-81.
PubMed
Lal G, Bromberg JS: Epigenetic mechanisms of regulation of Foxp3 expression. Blood. 2009 Oct 29;114(18):3727-35.
PubMed
Barnes MJ, Powrie F: Regulatory T cells reinforce intestinal homeostasis. Immunity. 2009 Sep 18;31(3):401-11.
PubMed
Bettini M, Vignali DA: Regulatory T cells and inhibitory cytokines in autoimmunity. Curr Opin Immunol. 2009 Dec;21(6):612-8.
PubMed
Elkord E: Novel therapeutic strategies by regulatory T cells in allergy. Chem Immunol Allergy. 2008;94:150-7.
PubMed

Product specific references:

Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC: The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells. Cytometry A. 2009 Dec;75(12):1040-50.
PubMed
Gavin MA, Torgerson TR, Houston E, DeRoos P, Ho WY, Stray-Pedersen A, Ocheltree EL, Greenberg PD, Ochs HD, Rudensky AY: Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6659-64.
PubMed
Variant
0.1 mg
A6-601-C100
In stock
310.00 USD

0.025 mg
A6-601-C025
Out of stock
155.00 USD
Variant
0.1 mg
11-261-C100
In stock
310.00 USD

0.1 mg
11-261-C100
In stock
155.00 USD

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