Exbio — Research products — Antibodies — Nucleus — Anti-DDIT4L Purified

Anti-DDIT4L Purified

Regulatory status

RUO

Antigen

DDIT4L

Clone

DDIT-03

Format

Purified

Reactivity

Human

Application

FC(IC), WB

Variant

0.1 mg

11-725-C100

In stock

198.00 USD

0.025 mg

11-725-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-725-C100

In stock

198.00 USD

0.025 mg

11-725-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody DDIT-03 recognizes DDIT4L / REDD2 protein, which belongs to intracellular stress-induced proteins involved in mediation of cell death.

Application

FC(IC), WB

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

N-terminal recombinant fragment of human DDIT4L (amino acids 2-98)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DDIT4L, REDD2, Rtp801L

Antigen description

DDIT4L (DNA-damage-inducible transcript 4-like), also known as REDD2 (regulated in development and DNA damage response 2) or RTP801L is a stress-inducted protein, which was shown to mediate monocyte cell death through a reduction in thioredoxin-1 expression, and is highly expressed in atherosclerotic lesions. Stimulation of DDIT4L expression in macrophages increases oxidized LDL-induced macrophage death.

Entrez Gene ID 115265

UniProt ID Q96D03

Western blotting analysis of DDIT4L expression in HEK293-DDIT4L transfectants and HEK293 cells using mouse monoclonal antibody DDIT-03.

General references:

Cuaz-Pérolin C, Furman C, Larigauderie G, Legedz L, Lasselin C, Copin C, Jaye M, Searfoss G, Yu KT, Duverger N, Nègre-Salvayre A, Fruchart JC, Rouis M.REDD2 gene is upregulated by modified LDL or hypoxia and mediates human macrophage cell death. Arterioscler Thromb Vasc Biol. 2004 Oct;24(10):1830-5.
PubMed

Corradetti MN, Inoki K, Guan KL: The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem. 2005 Mar 18;280(11):9769-72.
PubMed

Imen JS, Billiet L, Cuaz-Pérolin C, Michaud N, Rouis M: The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression. Free Radic Biol Med. 2009 May 15;46(10):1404-10
PubMed