Anti-DDIT4L PE

Anti-DDIT4L PE
Regulatory status
RUO
Antigen
DDIT4L
Clone
DDIT-03
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-725-C100
Ask for delivery term HERE
242.00 USD

0.025 mg
1P-725-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-725-C100
Ask for delivery term HERE
242.00 USD

0.025 mg
1P-725-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody DDIT-03 recognizes DDIT4L / REDD2 protein, which belongs to intracellular stress-induced proteins involved in mediation of cell death.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
N-terminal recombinant fragment of human DDIT4L (amino acids 2-98)
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
DDIT4L, REDD2, Rtp801L
Antigen description
DDIT4L (DNA-damage-inducible transcript 4-like), also known as REDD2 (regulated in development and DNA damage response 2) or RTP801L is a stress-inducted protein, which was shown to mediate monocyte cell death through a reduction in thioredoxin-1 expression, and is highly expressed in atherosclerotic lesions. Stimulation of DDIT4L expression in macrophages increases oxidized LDL-induced macrophage death.
Entrez Gene ID 115265
UniProt ID Q96D03
1P-725_FC_Histogram
Separation of DDIT4L transfected HEK-293 cells stained using anti-human DDIT4L (DDIT-03) PE antibody (concentration in sample 5 μg/ml, red-filled) from DDIT4L transfected HEK-293 cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as DDIT4L PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of DDIT4L transfected HEK-293 cell suspension.

General references:

Cuaz-Pérolin C, Furman C, Larigauderie G, Legedz L, Lasselin C, Copin C, Jaye M, Searfoss G, Yu KT, Duverger N, Nègre-Salvayre A, Fruchart JC, Rouis M.REDD2 gene is upregulated by modified LDL or hypoxia and mediates human macrophage cell death. Arterioscler Thromb Vasc Biol. 2004 Oct;24(10):1830-5.
PubMed
Corradetti MN, Inoki K, Guan KL: The stress-inducted proteins RTP801 and RTP801L are negative regulators of the mammalian target of rapamycin pathway. J Biol Chem. 2005 Mar 18;280(11):9769-72.
PubMed
Imen JS, Billiet L, Cuaz-Pérolin C, Michaud N, Rouis M: The regulated in development and DNA damage response 2 (REDD2) gene mediates human monocyte cell death through a reduction in thioredoxin-1 expression. Free Radic Biol Med. 2009 May 15;46(10):1404-10
PubMed
Variant
0.1 mg
1P-725-C100
Ask for delivery term HERE
242.00 USD

0.025 mg
1P-725-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-725-C100
Ask for delivery term HERE
242.00 USD

0.025 mg
1P-725-C025
Delivery 1 week
121.00 USD

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