Exbio — Research products — Antibodies — Nucleus — Anti-DAXX Purified

Anti-DAXX Purified

Regulatory status

RUO

Antigen

DAXX

Clone

DAXX-01

Format

Purified

Reactivity

Human

Application

WB, ICC

Variant

0.1 mg

11-351-C100

In stock

165.00 USD

0.025 mg

11-351-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-351-C100

In stock

165.00 USD

0.025 mg

11-351-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody DAXX-01 recognizes Daxx, a cytoplasmic death domain containing protein mainly expressed in fetal and adult human and mouse tissue.

Application

WB, ICC

Reactivity

Human

Immunogen

Recombinant C-terminal part (aa 558-740) of human Daxx.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DAP6, EAP1, BING2

Antigen description

Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions both in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).

Entrez Gene ID 1616

UniProt ID Q9UER7

Immunocytochemistry staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01), nuclei were stained with DAPI (blue). A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm

General references:

Jung YS, Kim HY, Kim J, Lee MG, Pouysségur J, Kim E: Physical Interactions and Functional Coupling between Daxx and Sodium Hydrogen Exchanger 1 in Ischemic Cell Death. J Biol Chem. 2008 Jan 11;283(2):1018-25.
PubMed

Shih HM, Chang CC, Kuo HY, Lin DY: Daxx mediates SUMO-dependent transcriptional control and subnuclear compartmentalization. Biochem Soc Trans. 2007 Dec;35(Pt 6):1397-400.
PubMed

Li Q, Wang X, Wu X, Rui Y, Liu W, Wang J, Wang X, Liou YC, Ye Z, Lin SC: Daxx cooperates with the Axin/HIPK2/p53 complex to induce cell death. Cancer Res. 2007 Jan 1;67(1):66-74.
PubMed

Croxton R, Puto LA, de Belle I, Thomas M, Torii S, Hanaii F, Cuddy M, Reed JC. Daxx represses expression of a subset of antiapoptotic genes regulated by nuclear factor-kappaB. Cancer Res. 2006 Sep 15;66(18):9026-35.
PubMed

Muromoto R, Ishida M, Sugiyama K, Sekine Y, Oritani K, Shimoda K, Matsuda T: Sumoylation of Daxx regulates IFN-induced growth suppression of B lymphocytes and the hormone receptor-mediated transactivation. J Immunol. 2006 Jul 15;177(2):1160-70.
PubMed

Product specific references:

Leal-Sanchez J, Couzinet A, Rossin A, Abdel-Sater F, Chakrabandhu K, Luci C, Anjuere F, Stebe E, Hancock D, Hueber AO: Requirement for Daxx in mature T-cell proliferation and activation. Cell Death Differ. 2007 Apr;14(4):795-806.
PubMed