Anti-Cyclin D1 Purified

Anti-Cyclin D1 Purified
Regulatory status
RUO
Antigen
Cyclin D1
Clone
CD1.1
Format
Purified
Reactivity
Human, Rat
Variant
0.1 mg
11-535-C100
In stock
180.00 USD

0.025 mg
11-535-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Application details
Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/ml; positive tissue: colon.
Western blotting: Recommended dilution: 1 μg/ml.
Immunocytochemistry: Recommended dilution: 1 μg/ml.
Immunoprecipitation: Recommended dilution: 1 μg/ml.
Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.
Flow cytometry: Intracellular staining, membrane permeabilization is required.
Reactivity
Human, Rat
Immunogen
Purified cyclin D1 protein
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CYC-D1, CCND1, Bcl-1, PRAD1, U21B31
Antigen description
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Entrez Gene ID 595
UniProt ID P24385

General references:

Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11(8):957-72.
PubMed
Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12(22):3499-511.
PubMed
Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282(6):3799-808.
PubMed
Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6
PubMed
Variant
0.1 mg
11-535-C100
In stock
180.00 USD

0.025 mg
11-535-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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