Exbio — Research products — Antibodies — Nucleus — Anti-Cyclin D1 Purified

Anti-Cyclin D1 Purified

Anti-Cyclin D1 Purified
Regulatory status
RUO
Antigen
Cyclin D1
Clone
DCS-6
Format
Purified
Reactivity
Rat, Mouse, Non-human primates, Human
Application
FC, IP, WB, IHC(F), IHC(P), ICC
Variant
0.1 mg
11-166-C100
In stock
130.00 USD
0.025 mg
11-166-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Application
FC, IP, WB, IHC(F), IHC(P), ICC
Application details
Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Flow cytometry: Recommended dilution: 3-12 µg/ml
Reactivity
Rat, Mouse, Non-human primates, Human
Negative species
Cat, Sheep
Immunogen
recombinant human cyclin D1 (amino acids 1-295)
Other names
CYC-D1, CCND1, Bcl-1, PRAD1, U21B31
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-166 FC
Separation of HeLa cells stained using anti-Cyclin D1 (DCS-6) purified antibody (concentration in sample 9 μg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11(8):957-72.
PubMed
Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12(22):3499-511.
PubMed
Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282(6):3799-808.
PubMed
Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6
PubMed

Product specific references:

Bosone I, Cavalla P, Chiadò-Piat L, Vito ND, Schiffer D: Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas. Neuropathology. 2001 Sep;21(3):155-61.
PubMed
Jain P, Giustolisi GM, Atkinson S, Elnenaei MO, Morilla R, Owusu-Ankomah K, Rafiq-Mohammed F, Matutes E, Wotherspoon A, Catovsky D: Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry. J Clin Pathol. 2002 Dec;55(12):940-5.
PubMed
Albrecht JH, Hansen LK: Cyclin D1 promotes mitogen-independent cell cycle progression in hepatocytes. Cell Growth Differ. 1999 Jun;10(6):397-404.
PubMed
Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998 Nov 15;12(22):3488-98.
PubMed
Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, Royds JA, Peston D, Hall PA, Hanby AM, Barnes DM, Shousha S, O'Hare MJ, Lu X: High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6380-5.
PubMed
Variant
0.1 mg
11-166-C100
In stock
130.00 USD
0.025 mg
11-166-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
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