Exbio — Research products — Antibodies — Nucleus — Anti-Cyclin D1 Purified

Anti-Cyclin D1 Purified

Regulatory status

RUO

Antigen

Cyclin D1

Clone

DCS-6

Format

Purified

Reactivity

Rat, Mouse, Non-human primates, Human

Application

FC-IC, IP, WB, IHC-F, IHC-P, ICC

Variant

0.1 mg

11-166-C100

In stock

147.40 USD

0.025 mg

11-166-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

11-166-C100

In stock

147.40 USD

0.025 mg

11-166-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Application

FC-IC, IP, WB, IHC-F, IHC-P, ICC

Application details

Immunohistochemistry: Recommended dilution: 5-10 μg/ml.
Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.

Reactivity

Rat, Mouse, Non-human primates, Human

Negative species

Cat, Sheep

Immunogen

recombinant human cyclin D1 (amino acids 1-295)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

Antigen description

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

Entrez Gene ID 595

UniProt ID P24385

Separation of HeLa cells stained using anti-Cyclin D1 (DCS-6) purified antibody (concentration in sample 9 μg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11(8):957-72.
PubMed

Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12(22):3499-511.
PubMed

Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282(6):3799-808.
PubMed

Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6
PubMed

Product specific references:

Bosone I, Cavalla P, Chiadò-Piat L, Vito ND, Schiffer D: Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas. Neuropathology. 2001 Sep;21(3):155-61.
PubMed

Jain P, Giustolisi GM, Atkinson S, Elnenaei MO, Morilla R, Owusu-Ankomah K, Rafiq-Mohammed F, Matutes E, Wotherspoon A, Catovsky D: Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry. J Clin Pathol. 2002 Dec;55(12):940-5.
PubMed

Albrecht JH, Hansen LK: Cyclin D1 promotes mitogen-independent cell cycle progression in hepatocytes. Cell Growth Differ. 1999 Jun;10(6):397-404.
PubMed

Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998 Nov 15;12(22):3488-98.
PubMed

Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, Royds JA, Peston D, Hall PA, Hanby AM, Barnes DM, Shousha S, O'Hare MJ, Lu X: High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6380-5.
PubMed