Anti-Cyclin D1 PE

Anti-Cyclin D1 PE
Regulatory status
RUO
Antigen
Cyclin D1
Clone
DCS-6
Format
PE
Reactivity
Rat, Mouse, Non-human primates, Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-166-C100
In stock
242.00 USD

0.025 mg
1P-166-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-166-C100
In stock
242.00 USD

0.025 mg
1P-166-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Application
Application details
Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
Reactivity
Rat, Mouse, Non-human primates, Human
Negative species
Cat, Sheep
Immunogen
recombinant human cyclin D1 (amino acids 1-295)
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CYC-D1, CCND1, Bcl-1, PRAD1, U21B31
Antigen description
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Entrez Gene ID 595
UniProt ID P24385
1P-166_FC_Profil Značení
Flow cytometry intracellular staining pattern of HUVEC cells stained using anti-Cyclin D1 (DCS-6) PE antibody (concentration in sample 15 μg/ml).
1P-166_FC_Histogram
Separation of HUVEC cells stained using anti-Cyclin D1 (DCS-6) PE antibody (concentration in sample 15 μg/ml, red-filled) from HUVEC cells stained using mouse IgG2a isotype control (MOPC-173) PE antibody (concentration in sample 15 μg/ml, black-dashed) in flow cytometry analysis (intracellular staining) of HUVEC cell suspension.

General references:

Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine-286 prevents its rapid degradation via the ubiquitin-proteasome pathway. Genes Dev. 1997 Apr 15;11(8):957-72.
PubMed
Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase-3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15;12(22):3499-511.
PubMed
Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3-kinase/AKT/GSK-3 beta pathway and is antagonized by insulin-like growth factor 1. J Biol Chem. 2007 Feb 9;282(6):3799-808.
PubMed
Mukherji A, Janbandhu VC, Kumar V: GSK-3beta-dependent destabilization of cyclin D1 mediates replicational stress-induced arrest of cell cycle. FEBS Lett. 2008 Mar 6
PubMed

Product specific references:

Bosone I, Cavalla P, Chiadò-Piat L, Vito ND, Schiffer D: Cyclin D1 expression in normal oligodendroglia and microglia cells: its use in the differential diagnosis of oligodendrogliomas. Neuropathology. 2001 Sep;21(3):155-61.
PubMed
Jain P, Giustolisi GM, Atkinson S, Elnenaei MO, Morilla R, Owusu-Ankomah K, Rafiq-Mohammed F, Matutes E, Wotherspoon A, Catovsky D: Detection of cyclin D1 in B cell lymphoproliferative disorders by flow cytometry. J Clin Pathol. 2002 Dec;55(12):940-5.
PubMed
Albrecht JH, Hansen LK: Cyclin D1 promotes mitogen-independent cell cycle progression in hepatocytes. Cell Growth Differ. 1999 Jun;10(6):397-404.
PubMed
Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R: Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1. Genes Dev. 1998 Nov 15;12(22):3488-98.
PubMed
Fredersdorf S, Burns J, Milne AM, Packham G, Fallis L, Gillett CE, Royds JA, Peston D, Hall PA, Hanby AM, Barnes DM, Shousha S, O'Hare MJ, Lu X: High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6380-5.
PubMed
Variant
0.1 mg
1P-166-C100
In stock
242.00 USD

0.025 mg
1P-166-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-166-C100
In stock
242.00 USD

0.025 mg
1P-166-C025
Delivery 1 week
121.00 USD

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