Exbio — Research products — Antibodies — Nucleus — Anti-Cyclin B1 Purified

Anti-Cyclin B1 Purified

Regulatory status

RUO

Antigen

Cyclin B1

Clone

V152

Format

Purified

Reactivity

Mammalian

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

11-871-C100

In stock

154.00 USD

Variant

0.1 mg

11-871-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.

Application

WB, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 2-5 µg/ml. Intracellular staining.

Reactivity

Mammalian

Immunogen

Recombinant His-tagged hamster cyclin B1

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.

Entrez Gene ID 891

UniProt ID P14635

Separation of JURKAT cells stained using anti-Cyclin B1 (V152) purified antibody (concentration in sample 5,0 μg/ml, GAM APC, red-filled) from JURKAT cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Yang Y, Xue K, Li Z, Zheng W, Dong W, Song J, Sun S, Ma T, Li W: c-Myc regulates the CDK1/cyclin B1 dependent‑G2/M cell cycle progression by histone H4 acetylation in Raji cells. Int J Mol Med. 2018 Jun;41(6):3366-3378.
PubMed

Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 21;25(12):2802-13.
PubMed

Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct;74(10):5602-8.
PubMed

Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2;153(1):137-48.
PubMed

Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 15;28(2):170-83.
PubMed