Anti-Cyclin B1 PE

Anti-Cyclin B1 PE
Regulatory status
RUO
Antigen
Cyclin B1
Clone
V152
Format
PE
Reactivity
Mammalian
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-871-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Mammalian
Immunogen
Recombinant His-tagged hamster cyclin B1
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.
Entrez Gene ID 891
UniProt ID P14635
1P-871_FC_Histogram
Separation of K562 cells stained using anti-human Cyclin B1 (V152) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from K562 cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 6 μg/ml, same as Cyclin B1 PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of K562 cell suspension.

Product specific references:

Yang Y, Xue K, Li Z, Zheng W, Dong W, Song J, Sun S, Ma T, Li W: c-Myc regulates the CDK1/cyclin B1 dependent‑G2/M cell cycle progression by histone H4 acetylation in Raji cells. Int J Mol Med. 2018 Jun;41(6):3366-3378.
PubMed
Wolf F, Wandke C, Isenberg N, Geley S: Dose-dependent effects of stable cyclin B1 on progression through mitosis in human cells. EMBO J. 2006 Jun 21;25(12):2802-13.
PubMed
Balsara ZR, Misaghi S, Lafave JN, Starnbach MN: Chlamydia trachomatis infection induces cleavage of the mitotic cyclin B1. Infect Immun. 2006 Oct;74(10):5602-8.
PubMed
Geley S, Kramer E, Gieffers C, Gannon J, Peters JM, Hunt T: Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint. J Cell Biol. 2001 Apr 2;153(1):137-48.
PubMed
Chan YW, Chen Y, Poon RY: Generation of an indestructible cyclin B1 by caspase-6-dependent cleavage during mitotic catastrophe. Oncogene. 2009 Jan 15;28(2):170-83.
PubMed
Variant
100 tests
1P-871-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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