Exbio — Research products — Antibodies — Nucleus — Anti-CTBP1 Purified

Anti-CTBP1 Purified

Regulatory status

RUO

Antigen

CTBP1

Clone

PAb (511)

Format

Purified

Reactivity

Mouse, Human

Application

IP, WB, ICC

Variant

0.1 mg

11-511-C100

In stock

198.00 USD

Variant

0.1 mg

11-511-C100

In stock

198.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Rabbit polyclonal

Specificity

The polyclonal antibody reacts with CtBP1, a 49 kDa transcriptional corepressor (intracellular antigen), that plays a critical role in regulating gene expression.

Application

IP, WB, ICC

Application details

Western blotting: Reducing conditions.
Immunocytochemistry: Recommended dilution 1 μg/ml, acetone / methanol fixation.

Reactivity

Mouse, Human

Immunogen

Bacterially expressed full-length human CtBP1

Concentration

1 mg/ml

Preparation

Purified by ligand affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

BARS, HADDTS

Antigen description

C-terminal binding protein 1 / brefeldin A (BFA)-dependent ADP-ribosylation substrate (CtBP1/BARS) is a highly conserved transcriptional corepressor, that plays a critical role in regulating gene expression patterns throughout development and in oncogenesis. CtBP regulates target gene transcription, in part, via recruitment of histone deacetylases involved in gene silencing and may act to coordinate histone modifications that regulate gene expression.

Entrez Gene ID 1487

UniProt ID Q13363

General references:

Valenta T, Lukáš J, Kořínek V: HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells. Nucleic Acids Res. 2003 May 1;31(9):2369-80.
PubMed

Nadauld LD, Phelps R, Moore BC, Eisinger A, Sandoval IT, Chidester S, Peterson PW, Manos EJ, Sklow B, Burt RW, Jones DA: Adenomatous polyposis coli control of C-terminal binding protein-1 stability regulates expression of intestinal retinol dehydrogenases. J Biol Chem. 2006 Dec 8;281(49):37828-35.
PubMed

Lopez-Garcia J, Periyasamy M, Thomas RS, Christian M, Leao M, Jat P, Kindle KB, Heery DM, Parker MG, Buluwela L, Kamalati T, Ali S: ZNF366 is an estrogen receptor corepressor that acts through CtBP and histone deacetylases. Nucleic Acids Res. 2006; 34(21):6126-36.
PubMed

Product specific references:

Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37.
PubMed