Exbio — Research products — Antibodies — Nucleus — Anti-BRG1 Purified

Anti-BRG1 Purified

Regulatory status

RUO

Antigen

BRG1

Clone

BRG-01

Format

Purified

Reactivity

Human

Application

Variant

0.1 mg

11-541-C100

In stock

198.00 USD

0.025 mg

11-541-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-541-C100

In stock

198.00 USD

0.025 mg

11-541-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Application

Application details

Western blotting: Recommended dilution: 10 μg/ml; positive control: HEK293 cells.

Reactivity

Human

Immunogen

Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CSS4, SMARCA4, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB

Antigen description

Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

Entrez Gene ID 6597

UniProt ID P51532

Western blotting analysis of Brg1 by BRG-01 antibody. A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression. B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.

General references:

Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281(51):38974-80.
PubMed

Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235(10):2722-35.
PubMed

Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A, Galderisi U: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 2007 Aug 15;120(Pt 16):2904-11.
PubMed