Anti-BRG1 Purified

Anti-BRG1 Purified
Regulatory status
RUO
Antigen
BRG1
Clone
BRG-01
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-541-C100
In stock
180.00 USD

0.025 mg
11-541-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.
Application
Application details
Western blotting: Recommended dilution: 10 μg/ml; positive control: HEK293 cells.
Reactivity
Human
Immunogen
Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CSS4, SMARCA4, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB
Antigen description
Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.
Entrez Gene ID 6597
UniProt ID P51532
11-541 WB
Western blotting analysis of Brg1 by BRG-01 antibody. A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression. B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.

General references:

Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281(51):38974-80.
PubMed
Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235(10):2722-35.
PubMed
Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A, Galderisi U: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 2007 Aug 15;120(Pt 16):2904-11.
PubMed
Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235(10):2722-35.
PubMed
Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281(51):38974-80.
PubMed
Variant
0.1 mg
11-541-C100
In stock
180.00 USD

0.025 mg
11-541-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD